FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_23.3410000000a095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_23.3410000000a095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC342.548725637181409No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT100.7496251874062968No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC90.6746626686656672RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC80.5997001499250375TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC70.5247376311844079No Hit
CCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTGAGCACTCT60.4497751124437781No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC50.3748125937031484No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG50.3748125937031484No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGG40.29985007496251875No Hit
GTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAA40.29985007496251875No Hit
ACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCT40.29985007496251875No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA40.29985007496251875No Hit
GTTCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTTGATTTTAG30.22488755622188905No Hit
GCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAAC30.22488755622188905No Hit
GTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTA30.22488755622188905No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTC30.22488755622188905No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA30.22488755622188905No Hit
CCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCT30.22488755622188905No Hit
GTACTAAACACCCTCTAATTATCCTAAGATAAAATCCAATCTGGCTCACGC30.22488755622188905No Hit
GTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAA30.22488755622188905No Hit
GTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCTGTCTCTTA30.22488755622188905No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGC30.22488755622188905No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGCTGTCTC30.22488755622188905No Hit
AACTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGAGTTCAAATC20.14992503748125938No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCCTGTCTCTTATACACA20.14992503748125938No Hit
GACTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGA20.14992503748125938No Hit
CCTCTAACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG20.14992503748125938No Hit
CTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGAGTTCAAATCGG20.14992503748125938No Hit
CTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATTCT20.14992503748125938No Hit
GTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTT20.14992503748125938No Hit
TTTTTAGGGTGTATGGATAGATACTTTGGGGGTAACAGGATTATTTTCTTT20.14992503748125938No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTCT20.14992503748125938No Hit
GATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAA20.14992503748125938No Hit
CTTATACAACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT20.14992503748125938No Hit
ATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGG20.14992503748125938No Hit
CTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGCTGTCTCTTATA20.14992503748125938No Hit
ATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTTC20.14992503748125938TruSeq Adapter, Index 27 (95% over 22bp)
GTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGC20.14992503748125938No Hit
CCTAAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGT20.14992503748125938No Hit
AAACAAAGCATTGCGATGGTCCCTGCGGATGCTAACGCAATGTGATTTCTG20.14992503748125938No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTC20.14992503748125938No Hit
TTTGAACTCAGCTCATGTTCAATTTTAATAGTCGAACAGACTATTAGCTGT20.14992503748125938No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATCTGTCTCT20.14992503748125938No Hit
GTTTCTTTATTGGGAACTTGTATGAGTGGAGTAATAGGTGCTTTTATGAAT20.14992503748125938No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGGGACAGA20.14992503748125938No Hit
AACATAATTAGAGTCAGGTTACTTGAAAGTGTAACCTAAATTAGGAGGTAA20.14992503748125938No Hit
ACATAAACCCTCTCTGAGAAGAGCGGGTAACCATATGAAAATTCTTCGTAA20.14992503748125938No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGCT20.14992503748125938No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers