FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_21.3410000000a07b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_21.3410000000a07b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1089
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC171.5610651974288337No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC80.7346189164370982No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT60.5509641873278237No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA50.4591368227731864No Hit
GACTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGA40.3673094582185491No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC40.3673094582185491RNA PCR Primer, Index 27 (95% over 21bp)
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG40.3673094582185491No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGAT40.3673094582185491No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATCTGTCTCTTATAC30.27548209366391185No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTCTTATACAC30.27548209366391185No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC20.18365472910927455No Hit
AAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGG20.18365472910927455No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGG20.18365472910927455No Hit
ATAATCCTGTTACCCCCAAAGTATCTATCCATACACCCTAAAAAAGCTGTC20.18365472910927455No Hit
GTGTATGGATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTC20.18365472910927455No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCCTGTCTCT20.18365472910927455No Hit
GTTGATACCACTGCCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACA20.18365472910927455No Hit
CCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTGAGCACTCT20.18365472910927455No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTGTCTCTTATAC20.18365472910927455No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTGTC20.18365472910927455No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCT20.18365472910927455No Hit
GTTTTATTATTGCGAACTTTTTTAGGGTGTATGGATAGATACCTGTCTCTT20.18365472910927455No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTCTGTC20.18365472910927455No Hit
TTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTT20.18365472910927455No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC20.18365472910927455TruSeq Adapter, Index 12 (95% over 21bp)
GATTTGAACTCAGCTCATGTTCAATTTTAATAGTCGAACAGACTATTAGTT20.18365472910927455No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA20.18365472910927455No Hit
ACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCT20.18365472910927455No Hit
TTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCTTAT20.18365472910927455No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGG20.18365472910927455No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA20.18365472910927455No Hit
TCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGCTGTCTCTTA20.18365472910927455No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)