FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_20.3410000000a06e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_20.3410000000a06e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC141.3435700575815739No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG60.5758157389635317No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC50.47984644913627633RNA PCR Primer, Index 27 (95% over 21bp)
AAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAATATTCTTGCATGGAAT50.47984644913627633No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAG40.3838771593090211No Hit
GCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAAC40.3838771593090211No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA40.3838771593090211No Hit
ACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCT40.3838771593090211No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC30.28790786948176583No Hit
GTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAA30.28790786948176583No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT30.28790786948176583No Hit
TAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTA30.28790786948176583No Hit
CCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTGAGCACTCT30.28790786948176583No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGG20.19193857965451055No Hit
CAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCTGTCTCT20.19193857965451055No Hit
GTGTATGGATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTC20.19193857965451055No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATCTGTCTCTTATAC20.19193857965451055No Hit
CCTCTAACTTTCGTTCTTGATTAATGAAAACATTCTTGGCCTGTCTCTTAT20.19193857965451055No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC20.19193857965451055No Hit
GATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTCTTATTTT20.19193857965451055No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCTTATACACATCTCC20.19193857965451055No Hit
CCTAAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGT20.19193857965451055No Hit
AGTTCTAACCATAAACGATGCCAACCAGCGATCAGTCTGAGTACTTTTATT20.19193857965451055No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA20.19193857965451055No Hit
AGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGAGGTAATA20.19193857965451055No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC20.19193857965451055TruSeq Adapter, Index 12 (95% over 21bp)
GGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAA20.19193857965451055No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGGGACAGA20.19193857965451055No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA20.19193857965451055No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGCTGTCTC20.19193857965451055No Hit
GTTTTATTATTGCGAACTTTTTTAGGGTGTATGGATAGATACTTTGGGGGT20.19193857965451055No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)