FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_2.34100000009f3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_2.34100000009f3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406059
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10420.2566129552601962No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9020.22213520695268418No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC8310.2046500631681603TruSeq Adapter, Index 12 (95% over 21bp)
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG8140.20046347944510526No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7600.18716491938363636No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7380.18174698750674162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.16573946150682536No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC6060.1492393962453732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.14874685698383733No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5890.1450528125223182No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5670.13963488064542345No Hit
CTCTAACGGCGTACCTTTTGCATGATGGGTCAGCGAGGAAATGGGAAGAGC5230.12879901689163398No Hit
CTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCTCT4920.12116465833782776No Hit
TGGCTAGATCGATCGGTTTCGGGTCAAATAGGAAGAACTAGAAGATTCCAC4820.11870196203014832No Hit
ATCTAGCCATGAGCAGGTTGAAGAGAGCTCTAACAGGCCTTGGAGGACCGA4760.11722434424554067No Hit
CCGTTAGAGTGAGTGCGCTTGACTACTCCTTCGACTGATTGTTCGCATCGG4710.11599299609170097No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT4710.11599299609170097No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4370.10761982864559091No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC4320.10638848049175119No Hit
ATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAA4310.10614221086098326No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATG4170.10269443603023207No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA4110.10121681824562441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT356.2394847E-638.57037444
CATTTAA250.002093091836.0433961
CGTATGC900.034.99904341
ATGCCGT900.034.99904344
ACCTATA1200.033.7906881
GCAATCT801.8189894E-1233.74907734
CTCGTAT950.033.1569939
TCGTATG950.033.1569940
TACTATG300.005144071329.999182
TGCTAGG300.005144071329.999182
ATACAAG300.005144071329.999183
TATGCCG1100.028.6355843
CCCACGA1100.028.6355819
GTATGCC1100.028.6355842
CAATCTC1100.028.6355835
TTGCAAT952.3646862E-1128.42027732
TGCAATC952.3646862E-1128.42027733
TACTTGC1004.5474735E-1126.99926229
ATACCCG1350.026.6659375
CACGAGA1200.026.24928321
Produced by FastQC (version 0.11.2)