FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_19.3410000000a052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_19.3410000000a052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC312.995169082125604No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC111.0628019323671498RNA PCR Primer, Index 27 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT60.5797101449275363No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTC60.5797101449275363No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTC50.4830917874396135No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA50.4830917874396135No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG50.4830917874396135No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC40.3864734299516908TruSeq Adapter, Index 12 (95% over 21bp)
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCCTGTCT40.3864734299516908No Hit
CCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGAT30.2898550724637681No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC30.2898550724637681No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACCTGTCTCTTA30.2898550724637681No Hit
GATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTCTTATTTT30.2898550724637681No Hit
GTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAA30.2898550724637681No Hit
GTAATAAACTTCCCCTAAAATAAGAACTTCAAAAGAAAATAATCCTGTTAC30.2898550724637681No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA30.2898550724637681No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGGGACCTG30.2898550724637681No Hit
CAATTTTAATAGTCGAACAGACTATTAGTTTGAAAAGCTACCCCCAACTAA20.1932367149758454No Hit
GTTCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTTGATTTTAG20.1932367149758454No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAG20.1932367149758454No Hit
AAATAATCCTGTTACCCCCAAAGTATCTATCCATACACCTGTCTCTTATAC20.1932367149758454No Hit
GCTTAAAGAGGAGAAGACATTATGAGTAAGAGGAACGCAATGATAGCGTCG20.1932367149758454No Hit
GTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCTGTCTCTTATAC20.1932367149758454No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCACTGTCTCTTATAC20.1932367149758454No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGACGT20.1932367149758454No Hit
CCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCT20.1932367149758454No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCT20.1932367149758454No Hit
CCTAAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGT20.1932367149758454No Hit
CAGTAACATTCAGCTTCAACAATCCTCCTAACCATTGTACAGACAGTAGAG20.1932367149758454No Hit
CTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATCTGTCTCTTATACACA20.1932367149758454No Hit
GGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAA20.1932367149758454No Hit
GGTTTCCCCCGACTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCAC20.1932367149758454No Hit
CTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGCTGTCTCTTA20.1932367149758454No Hit
ACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCT20.1932367149758454No Hit
TTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCTTAT20.1932367149758454No Hit
GTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCTGTCTCTTA20.1932367149758454No Hit
CCTAAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTG20.1932367149758454No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGG20.1932367149758454No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)