FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_17.3410000000a038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_17.3410000000a038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC211.9626168224299065No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT90.8411214953271028No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG70.6542056074766356No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA50.46728971962616817No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC50.46728971962616817TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC40.3738317757009346No Hit
CCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTGAGCACTCT40.3738317757009346No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA30.2803738317757009No Hit
GTACGGGATCGGGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGC20.1869158878504673No Hit
GTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAA20.1869158878504673No Hit
AAGCAGGCATTTCGCCTAAATATTCTTGCATGGAATAATGGAATAAGATCT20.1869158878504673No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTC20.1869158878504673No Hit
GATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAA20.1869158878504673No Hit
GTGTATGGATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTC20.1869158878504673No Hit
GACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCTGTCTCTTATAC20.1869158878504673No Hit
CTCTAATTATCCTAAGATAAAATCCAATCTGGCTCACGCCGATTTGAACTC20.1869158878504673No Hit
GTTCTCATTTGAATATTTGCTACTACCACCAAGATCCTGTCTCTTATACAC20.1869158878504673No Hit
CTTATAACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT20.1869158878504673No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAAGGCGATCAGA20.1869158878504673No Hit
TTCTTGCATGGAATAATGGAATAAGATCTAGGTCTGTCTCTTATACACATC20.1869158878504673No Hit
CTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGCTGTCTCTTA20.1869158878504673No Hit
ATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTG20.1869158878504673No Hit
AAATAATCCTGTTACCCCCAAAGTATCTATCCATACACCCTAAAAAAGTTC20.1869158878504673No Hit
ACATAAACCCTCTCTGAGAAGAGCGGGTAACCATATGAAAATTCTTCGTAA20.1869158878504673No Hit
AAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAATATTCTTGCATGGAAT20.1869158878504673No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)