FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_16.3410000000a02b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_16.3410000000a02b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC242.2222222222222223No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG80.7407407407407408No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC70.6481481481481481No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT70.6481481481481481No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC60.5555555555555556RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC60.5555555555555556No Hit
GACTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGA40.3703703703703704No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA40.3703703703703704No Hit
GTAATAAACTTCCCCTAAAATAAGAACTTCAAAAGAAAATAATCCTGTTAC40.3703703703703704No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATCTGTCTCTTATA30.2777777777777778No Hit
ACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCT30.2777777777777778No Hit
GATAGATACTTTGGGGGTAACAGGATTATTTCTGTCTCTTATACACATCTC30.2777777777777778No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA30.2777777777777778No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGAT30.2777777777777778No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
ACCGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTTA20.1851851851851852No Hit
CTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGAGG20.1851851851851852No Hit
TACTCAGACTGATCGCTGGTTGGCATCGTTTATGGTTAGAACTAGGGCGGT20.1851851851851852No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACC20.1851851851851852No Hit
GATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAA20.1851851851851852No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT20.1851851851851852No Hit
CCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAA20.1851851851851852No Hit
TCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTTGATTTTAGTT20.1851851851851852No Hit
GGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAA20.1851851851851852No Hit
CGTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGA20.1851851851851852No Hit
ATGCCTGAGCGGGGTAAAGCCAGAGGAAACTCTGGTGGAAGCCCGCAGCGA20.1851851851851852No Hit
GCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGA20.1851851851851852No Hit
AAATAATCCTGTTACCCCCAAAGTATCTATCCATACACCCTAAAAACTGTC20.1851851851851852No Hit
ATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTG20.1851851851851852No Hit
GTGTATGGATAGATACTTTGGGGGTAACAGGATTATTTCTGTCTCTTATAC20.1851851851851852No Hit
ACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTATT20.1851851851851852No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)