FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_15.3410000000a01e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_15.3410000000a01e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329313
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAG348011.4940456692595627No Hit
GTTTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAA188830.8106682098970813No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATC181290.7782981505705759No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGA162910.6993907645730737No Hit
GACTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGA131130.5629556869343021No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCA129980.5580186089203126No Hit
CCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTGAGCACTCT128110.5499904907584339No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTC122680.5266788963097703No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGAT120020.5152592202078466No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAG117450.5042259241244091No Hit
ACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCT105360.4523222083077714No Hit
AAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAATATTCTTGCATGGAAT97860.4201238734339267No Hit
GGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAA91220.39161761429228276No Hit
CGTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGA69270.29738382089483034No Hit
CTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTC69050.2964393364051976No Hit
GATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTCTTATTTT68490.29403519406795053No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGGGACAGA68330.2933482962573085No Hit
ATCTTATTCCATTATTCCATGCAAGAATATTTAGGCGAAATGCCTGCTTTG67110.28811070045116305No Hit
GCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAAC66480.2854060403217601No Hit
CTATTAGTTTGAAAAGCTACCCCCAACTAAAATCAAAAGCCAACATCGAGG63750.2736858464276806No Hit
GATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGCCTAAA61860.26557186603947175No Hit
ACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTAGG61650.2646703126630041No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTC61140.26248082589158267No Hit
CTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGAGTTCAAATCGG59840.2568997811801162No Hit
GTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGC59460.2552683988798414No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC57130.24526544951236692No Hit
GTAATAAACTTCCCCTAAAATAAGAACTTCAAAAGAAAATAATCCTGTTAC55750.23934095589557952No Hit
GATTTGAACTCAGCTCATGTTCAATTTTAATAGTCGAACAGACTATTAGTT55490.23822474695328624No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATTAAAAGCTGTCTC55020.2362069846345253No Hit
TTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTT54800.23526250014489247No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTCTTATAC54590.23436094676842484No Hit
GTCTGTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAA54240.23285835780764544No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCTTATACACATCTCC53910.23144163107319624No Hit
GTCCCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTA52480.2253024818905832No Hit
GTGTATGGATAGATACTTTGGGGGTAACAGGATTATTTTCTTTTGAAGTTC51680.22186799283737307No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATCTGTCTCTTATAC50450.21658746591806255No Hit
GTACTAAACACCCTCTAATTATCCTAAGATAAAATCCAATCTGGCTCACGC48340.20752900104022087No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTGTC48170.20679917211641374No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCTGTCTCTTATACACATCTCC43560.18700792894729046No Hit
AACTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGAGTTCAAATC42680.18322999098875936No Hit
CTAATTATCCTAAGATAAAATCCAATCTGGCTCACGCCGATTTGAACTCAG42070.18061119308568663No Hit
CCTTTTAATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGAT39810.17090876151036807No Hit
CAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATTTA39560.1698354836812399No Hit
GTATCTATCCATACACCCTAAAAAAGTTCGCAATAATAAAACCTGTCTCTT39460.16940617254958867No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGCT37430.160691156577068No Hit
TTTTTAGGGTGTATGGATAGATACTTTGGGGGTAACAGGATTATTTTCTTT37070.15914563650312347No Hit
CCTAAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGT36770.15785770310816966No Hit
GTTCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTTGATTTTAG36600.1571278741843625No Hit
GTTTTATTATTGCGAACTTTTTTAGGGTGTATGGATAGATACCTGTCTCTT35860.15395097181014317No Hit
ATCGGGATCTTTACTTTGAAAAAATTAGAGTGCTCAAAGCAGGCATTTCGC34870.14970079160679567No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAAGGCGATCAGA34230.14695320036422757No Hit
CTTTTGAAGTTCTTATTTTAGGGGAAGTTTATTACCTCGATGTTGGCTTTT32720.1404706022762935No Hit
ATCATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCCTGTCT32250.13845283995753255No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAACTGTCTCTTAT31610.13570524871496445No Hit
GTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCTGTC31520.13531886869647833No Hit
TTATTACCTCGATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAAAC31450.13501835090432243No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGATCTGTCT30930.13278593301973587No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACA30430.13063937736147954No Hit
GTTTTATTATTGCGAACTTTTTTAGGGTGTATGGATAGATACTTTGGGGGT30420.13059644624831443No Hit
CCCTCTAATTATCCTAAGATAAAATCCAATCTGGCTCACGCCGATTTGAAC30030.12892213283487447No Hit
GAATAATGGAATAAGATCTAGGTTCTATTCTGTTGGTCCTGTCTCTTATAC29870.12823523502423245No Hit
ATTTTAATAGTCGAACAGACTATTAGTTTGAAAAGCTACCCCCAACTAAAA29850.12814937279790223No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTGTCTCTTATACAC29550.12686143940294844No Hit
GACCAACAGAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATAT29400.12621747270547154No Hit
AAATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCTGTTGG29080.12484367708418748No Hit
GATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGCTGTCTCT28600.12278298365226141No Hit
TTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCTTAT28170.12093694578616099No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCT28070.12050763465450971No Hit
AAGCAGGCATTTCGCCTAAATATTCTTGCATGGAATAATGGAATAAGATCT27630.11861866567524416No Hit
GTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAATGCTGTCTCTTATACAC27020.11599986777217144No Hit
GAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTGTCTCTTATAC26800.11505538328253867No Hit
CATTAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCCTGTCTCT26400.11333813875593361No Hit
GAATAGAACCTAGATCTTATTCCATTATTCCATGCAAGAATATCTGTCTCT26260.11273710317162185No Hit
ATATTCTTGCATGGAATAATGGAATAAGATCTAGGTTCTATTCCTGTCTCT26110.11209313647414494No Hit
CTAATAGTCTGTTCGACTATTAAAATTGAACATGAGCTGAGTTCAAATCCT26080.11196434313464956No Hit
GTATTTTGACTGTGCTAAGGTAGCGAAGTCATTTGTTTCTTTATTGGGAAC26020.1117067564556588No Hit
TAACTGAGGTTCAAAAGACCAACAGAATAGAACCTAGATCTTATTCCATTA25760.11059054751336551No Hit
ATGTTGGCTTTTGATTTTAGTTGGGGGTAGCTTTTCAAACTAATAGTCTGT24280.10423674276492682No Hit
ATGGAATAAGATCTAGGTTCTATTCTGTTGGTCTTTTGAACCTCAGTTAAT23490.10084518482488185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7300.039.45073745
GTTTTAT20200.034.245861
TCGCGCG754.7293724E-1132.9988989
ATGCCGT8600.032.70239644
TATGCCG8850.032.0328343
CGAACTT20000.031.83643513
TTGCGAA20200.031.63260810
TGCGAAC20250.031.33228711
CTAGGTG651.4053512E-831.1528074
TATCTCG9150.030.7366836
GTATGCC9150.030.7366842
TCGTATG9150.030.7366840
CTCGTAT9200.030.56963239
CCCACGA9200.030.56963219
CGTATGC9300.030.24092541
CCACGAG9300.030.24092520
TTATTAT21500.030.033884
GGCGTTA300.005149171729.99899913
TATTATT21700.029.3423375
ACGAGAC9800.029.1571922
Produced by FastQC (version 0.11.2)