FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_14.3410000000a001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_14.3410000000a001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387877
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC21760.15678622817439875TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2600.030.28763843
GCCCACG3250.023.5378218
CGTATGC3350.023.50682341
CCCACGA3450.022.82546619
TCGCGCG3250.022.1532449
TCGTATG3650.021.57475540
ATGCCGT3900.021.34557344
CGCGCGC3500.021.21370910
GTATGCC3900.020.76866742
GGGGCTA3600.020.6244436
CTAGACT1151.5963997E-719.5646844
GTCGCGC3800.018.9468548
GGCTAGT3850.018.70078838
CGTCGGG2550.018.52890814
GCGCGCT4800.018.28075211
GTATCAA23150.017.9048141
GCCTCGA2650.017.82970432
CTCGTAT4450.017.69614839
TACCCGG2800.017.678096
CGGGGCA3450.017.60821717
Produced by FastQC (version 0.11.2)