FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_13.34100000009fe9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_13.34100000009fe9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424631
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.22537214664025942No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC7530.17733043513073704RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA6260.14742211473020103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6130.14436063311439815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.12293026180377788No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5070.11939778301631299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG250.002090188236.053971
CGTATGC1300.031.15278441
GCCCACG1400.030.53467818
GTATGCC1400.028.9275942
TATGCCG1450.027.93008243
CGAGACG1300.027.69136423
ATGCCGT1600.026.71784244
GTATTAG608.0810805E-626.2893541
TAATACA608.177816E-626.2491074
TCGTATG1550.026.12814340
CAGGCGC551.3665343E-424.544624
CCCACGA1650.024.54461919
TGCTATC1600.023.90543733
CACGAGA1700.023.8227221
TATCTCG1750.023.14206936
TCCGAGC1900.022.49923513
ATAATAC500.002262723222.4992353
ACGAATT1600.022.49923527
CTCGTAT1850.021.89114839
TCTCGTA1850.021.89114838
Produced by FastQC (version 0.11.2)