FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_12.34100000009fdc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_12.34100000009fdc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC22.197802197802198TruSeq Adapter, Index 12 (95% over 21bp)
CACTGAGTATTTAGGCTTACCGGGTGGTCCCGGCAGATTCACAGCAGATTC11.098901098901099No Hit
CGCCGCTACTAACGGGGTCTCGGTTGATTCCCCTTCCTTTAGCTACTTAGA11.098901098901099No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.098901098901099No Hit
GCTATAGTTTCGGGTATACTTCTTTCAAGAAGAAAGTGAAAAAGGATTGTA11.098901098901099No Hit
TGGGTGTACTATACTACTACTACCTGTAATCTTTTTCCTGTCTCTTATACA11.098901098901099No Hit
GTATCAACGCAGAGTACGGGACGCTTGTGGAGACGTCGCTGCCGTGATCGT11.098901098901099No Hit
CTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGACTTCCG11.098901098901099No Hit
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAATCCCC11.098901098901099No Hit
CTTATCGACGGAGTCGTTTGGCACCTCGATGTCGACTCATCACATCCTGGG11.098901098901099No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGATCC11.098901098901099No Hit
CCTCAAAACGAGTGGTATTTCTTTAGCCACAAGGACAAGAAATATCCGACT11.098901098901099No Hit
TACTACAGGACGTTTACCTAGCCAACATTTCGATCCGGCTCTACCCAAACT11.098901098901099No Hit
TGGCTAGATCGATCGGTTTCGGGTCAAATAGGAAGAACTAGAAGATTCCAC11.098901098901099No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTTCTCCGCTTATTGATATGCTT11.098901098901099No Hit
GATTGGGGCTGCATTCCCAAACAACCCGACTCGTAGCCCGTACCTGTCTCT11.098901098901099No Hit
TGCTTGCTTAGCTTTGAGGAAGTTAAAAAAGACGGATGTTTTGTAAAAGCC11.098901098901099No Hit
CTTAGGGACCTTAGCGTACGATCTGGGCTGTTTCCCTCTCGCCTTTGGATC11.098901098901099No Hit
TCCTGGGTGTGCTGCCCTATCTCATCCTGCTCGCCTGCCCGCTCATGCACC11.098901098901099No Hit
GGTCTAGCGGATCACCGCATATTGGTGGTGGTGCTAAAGTTAGGGAGGCGT11.098901098901099No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC11.098901098901099No Hit
ACACTGTGGAGATCCTCCAGAATGTGACTCAATATCTGTCCCTTGCGAAAA11.098901098901099No Hit
GGATTCATGTGCGTGTGGAGCATGTGCAGCAATCAAGATGTGCTGAGGAGT11.098901098901099No Hit
GTCTTCAACTGGTTCATGGCGTTCAAGTTCCTGGTAGCTTCTTCCTCGCTC11.098901098901099No Hit
AAGATATGATGATTGGTTTAGGTACCAATTTTTGGGCCAATTCCCTCCCCG11.098901098901099No Hit
CCACTGCTCTTTCAAATTCAAAATGTTATCTGTGTGTAAATAGCTTTGGTA11.098901098901099No Hit
CTTGTGAATGTCTCAAAATTGCTTTTAAACAAACCAGCCAATTTCAACAAT11.098901098901099No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAGAAAAAATGT11.098901098901099No Hit
GGCAAAAAGTTTTGAGTTCTAACCACCATATAATGTACATACATGCTTGCT11.098901098901099No Hit
CGTCTTCTCCATTTACGGCTCCGTCCTCACCGTTAAGATTGTCAATGACCG11.098901098901099No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTA11.098901098901099No Hit
TACCAGGGCTTGACATGCCGCGAATCCTCTTGAAAGAGAGGGGTGCCTTCG11.098901098901099No Hit
GTCTAGGGGGCTTATCGGCTTACCGAAATCAGCCAAACTCCGAATGCTGGC11.098901098901099No Hit
GAAGAGCACCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGA11.098901098901099No Hit
GTGCTCTACCTCGGGCCATCGACATCATACGCTCTACTGAAATAGATTTCG11.098901098901099No Hit
ACTCATGCCGGCATCCGCACTTCAGTCGGCTCCACCACTCCTTCCGGTATG11.098901098901099No Hit
GAAGGTGAGAGTCCTGTAGGCGAACGAGCGGTTGACTGTACTGGCACCTGA11.098901098901099No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11.098901098901099No Hit
CCGTTACAACCAACGGCGTGGACGTGACAGCTGGTGAATACTCTGCTTTCT11.098901098901099No Hit
GTGTAACGACTGCCCCGCTGTCTCCGACATGGACCCGGTGAAATTGAATTC11.098901098901099No Hit
GAAGAGAATCAAAGTAATCTCTATGAAGCATCTGTCCAAGCATCACAAACA11.098901098901099No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGCTGAC11.098901098901099No Hit
GCTGTTTCTCGTCTCGGTGGACGATCTGCTTTCGTGGGAAAACTTGGAGAC11.098901098901099No Hit
CCATGGATCTCCGATCGGGAAACCGTATCCAAGCTCCGTGGCTAGTCTGCG11.098901098901099No Hit
ATGGAATGGTGGCTCATGTCAGAAGAAGCACCATCCCCCATTTTCAGGTGG11.098901098901099No Hit
GTCTTCGTCCAAGTCGGAGACGCTTACTCCGATCATAACTGTTGGGAAAGG11.098901098901099No Hit
ATCTTGGGGCTTAACCCTGAGCGTGCTTTCGATACGGGTTGACTTGAGGCT11.098901098901099No Hit
AAAAAAAAGTACTCTGCGTTGATACCACCCGTACTCCTGTCTCTTATACAT11.098901098901099No Hit
CCCCTAGCCACAAGTCATCCCCGTATTTTGCCACATACGTGGGTTCGGTCC11.098901098901099No Hit
TAGTAGTAGTTGGAATGTGAAATAGGGTTTATGCTTTTGGGTATCTTTGAG11.098901098901099No Hit
GAGTATACTAGGGCGCTTGAGAGAACCATGTCGAAGGAACTCGGCAAAATG11.098901098901099No Hit
GTAGGAGGGCGCGGCGGTCGCTGCAAAACCTAGGGCGCGAGCCCGGGCGGA11.098901098901099No Hit
AACCAACCATAAGTAGCAAAACCAAAAGAAGAGTCCTAATCCGAAACCATC11.098901098901099No Hit
GTTGTGACCCTTTCCACGAAGACCACGATTCTTCTTTCCTTCGGAGGTGAG11.098901098901099No Hit
GATAATATCCGACGAAGTATATGATCATGTTGTATACTGTCTCTTATACAC11.098901098901099No Hit
GTGGAAGATGTATGGAAATAATAACAAGAAGTCTATCAACATCACGAGTAT11.098901098901099No Hit
AGCTCAGACTGTATGAAACACAAACCTCGTGATACAAGACTGATTAAGAGA11.098901098901099No Hit
GTATGATTTCCTGCCATGAGCTAGCGTTCCTAGCAAAACCATCAGCTAGGT11.098901098901099No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT11.098901098901099No Hit
CTTCGAAAGGGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTTGAC11.098901098901099No Hit
GAAAATAAGTAACCGGAGACATAAACACAAGATAAAACTTGATCTCTCACG11.098901098901099No Hit
GGGCTAGGGTGGCCCCATTTCGCCTTACCAACCCCAGGGAAACTCCGAATA11.098901098901099No Hit
GCGACGGACCGGGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAGGGTGAGA11.098901098901099No Hit
TCCTGGTTCTGGACTGGCTCATCGGCTTATAAACCTGACTCTTGGATCATT11.098901098901099No Hit
GGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGTAAAGCCAGAGGAAAC11.098901098901099No Hit
CTTGAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGTGAGT11.098901098901099No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGAT11.098901098901099No Hit
CCGTCGCACTCATCGGAATATTCCATTGGCACCGTACTACCCTGAAATCAT11.098901098901099No Hit
CCCTAAGCAATCACTTAGTGGAAAAGGAAGTGATCGAGCGATGACAACCAG11.098901098901099No Hit
TCTCACGGCTTTTGCAACTTTCTCCTTCGAACCTAACCTCCCCACTAACCA11.098901098901099No Hit
ACCCTGGACGCTGTTGGGATATTGTTGACAAATACAAGGTTTCAATATTTT11.098901098901099No Hit
GGTGCAGGTAGTCCGCATCTTCACAGACATGTCTATTTCACCGAGCCTCTC11.098901098901099No Hit
TCCCGCCACTATGCCATTCCCCGTTTTGGAAATGATCGGGAGTCGAAGTCC11.098901098901099No Hit
CTTCGTTTAAGTTGCCTAAACAGAGTCCTCTCTCTCACCGCATTTTCAGAT11.098901098901099No Hit
CTCCACAAGTGTTCAATGAGCTGAGTTTCGGGAACCATGGGGGCTGTTGTG11.098901098901099No Hit
GTTGAACTGTGGTTCTGAAAGACGTGGAGTCTAAAACCCTTTACTCTAATG11.098901098901099No Hit
AATGAATTTGGCTCCACGTCACCATCCAATGGCCTTGGGAATGATTTATGA11.098901098901099No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAAC11.098901098901099No Hit
AGGCAGGAAGATCCCTTGAGCCTGGAGTTCAAGGTTGCAGCAAGCTATGAT11.098901098901099No Hit
CGTATACAGAGAGCCAGAGATCGTTTCTGAAAACTTGGGTATATTTTCTTA11.098901098901099No Hit
GTATCAACGCAGAGTACGGGTAAAAAAAAAAAAAAAAACAAAACTCTCTTA11.098901098901099No Hit
TAAGAGCACCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGA11.098901098901099No Hit
ACCTGGAAGTGCCTTCATATCAGCCACTAGCGAATAAGAATGAAGAAGATG11.098901098901099No Hit
GTCTCGATCTGCCGCTGCGACGTCAGCCGAAACTGGTTGCGCGGAAACGGA11.098901098901099No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG11.098901098901099No Hit
GAGGTATGTGTGCTTAGTTGCATGATGATGAATGATATAATAGAAGCATAC11.098901098901099No Hit
CCGCAGAGCTCCTCGAAACGGGCACGAGTGATCGAGGTGTAGAAGCTGTCT11.098901098901099No Hit
GATCTTGTCATCGACAGGCTTTGAACAATCAATGGTGAAGGAAACTCCCTT11.098901098901099No Hit
TTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACA11.098901098901099No Hit
CCTAAATGCATTTCGGGGAGAACCAGCTATCACGGAGTTTGATCTGTCTCT11.098901098901099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGA50.045.032
AGGGGAT50.045.08
GGTTGAC50.045.045
AACCGGG50.045.031
CCGGAAC50.045.027
AAATTCC50.045.022
AATTCCG50.045.023
TTCCGGA50.045.025
TAAAATT50.045.020
GCGGTTG50.045.043
TCGAAAG50.045.03
TTCGAAA50.045.02
GGATCCG50.045.011
AAGGGGA50.045.07
CGTGGCG50.045.039
TCCGGTT50.045.014
GGTTAAA50.045.017
CGGAACC50.045.028
TCCGGAA50.045.026
GACGTGG50.045.037