FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_2_10.34100000009fb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_2_10.34100000009fb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC32.083333333333333No Hit
CTTAAACTCAGCGGGTAATCCCGCCTGACCTGGGGTCGCCCGTACTCTGCG21.3888888888888888No Hit
CTTAGGGACCGATTCACTCTGCGTAGATTGACTGAACGCAGAAAGCCTTCC10.6944444444444444No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA10.6944444444444444No Hit
GTGTCGCCGCTCTCGGAGGTCTGGCTTAGCGCGCGGTGGCGTCCGTACTCG10.6944444444444444No Hit
TCTGAGGACGAGGCGCTCGTGAAATACCCGCAACTGAGCAAGCTGTAAAGA10.6944444444444444No Hit
GGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCG10.6944444444444444No Hit
TATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTT10.6944444444444444RNA PCR Primer, Index 27 (100% over 21bp)
GTAACAATACCGTGTTCAATGGGGTATTTGAGTGTTAAGATACCACGTTTA10.6944444444444444No Hit
CGGTACTTGTACGCTATCGGTCATTGAGGAATACTTAGGCTTAGAGGGTGG10.6944444444444444No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAATA10.6944444444444444No Hit
ACCCCACACGGGTTAACCTCGCGACGTGCCGCTGACTCGCAGGCTCATTCT10.6944444444444444No Hit
TGGCTAGATCGATCGGTTTCGGGTCAAATAGGAAGAACTAGAAGATTCCAC10.6944444444444444No Hit
GGATGAACCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAAC10.6944444444444444No Hit
GACCCATACCGAGGCGGCTGATGCTAACCACCCGAGCTGTCACCGCACCCT10.6944444444444444No Hit
CCCTATTTCATGTAAGTGCTCACTAAACAGTTACCACTTACTGTGACAAGA10.6944444444444444No Hit
CTCTTCAACCTGCTCATGGCTAGATCGATCGGTTTCGGGTCAAATAGGAAG10.6944444444444444No Hit
AACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACG10.6944444444444444No Hit
ACGACGCACCACGCCGTGAACAAGGCACACTGTTCCTCCTCATAGCACCGT10.6944444444444444No Hit
ACTTAGGCCCGGTCCGTCGCTGAGGACGCTTCTCCAGACTACAATTCGAAC10.6944444444444444No Hit
TTCCAGCACCACACTGTGCCCGTCTTCGCTGACGAAACGGCGGTGGTACAC10.6944444444444444No Hit
GTACGGGCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCA10.6944444444444444No Hit
TTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGA10.6944444444444444No Hit
GAGCAAAATAGTCAGTCTTCTCCTATTTGCCCTGTCTCTTATACACATCTC10.6944444444444444No Hit
AATCAAAGCAGTTAAAGAAGCAGCTGATGCAAAATTAGCAGATGTTAAGGC10.6944444444444444No Hit
GAATAGCATTCATCCTTTGATGCCTACTTCCACTCATCACATAACCAAGAT10.6944444444444444No Hit
CTTTAAAGATCAGACAGAGATTTGAGCAGAGACTATGTAAAGAGTTGCTTT10.6944444444444444No Hit
CCTACGTACTGTTGTATTGTAGCGTGATGTTTTCCCACTGCTTTCTGGCTT10.6944444444444444No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAATGT10.6944444444444444No Hit
ACCCCGCGCAGGGCCTGCACCTCGGGGCTGCCAATGCAGCTGGTGGGGGCC10.6944444444444444No Hit
GTATCAACGCAGAGTACGGGAAGTATGTATTATATATCTGTCTCTTATACA10.6944444444444444No Hit
ATCCCTACCTGATCCGAGGTCAACCCCGTACTCTGCGTTGATACCTGTCTC10.6944444444444444No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA10.6944444444444444No Hit
GATCACGGGCCAAGGTTAGAACCATCAACAAACCAGGGTGGTATTTCAAGG10.6944444444444444No Hit
GTCAAAGATGATGTTGATGATAAAGAACAAGATGCCAAAAGACAAAGTATG10.6944444444444444No Hit
GGCCTGTTAGAGCTCTCTTCAACCTGCTCATGGCTAGATCGCTCGGTTTCG10.6944444444444444No Hit
TACATACAGAGCACATGTGCTACCTCCGGAGAAGGGCTTTATGAGGGACTC10.6944444444444444No Hit
GTATCAACGCAGAGTACGGGACGGCGGAGGCGGTGGCCGTCCAATCCGGAG10.6944444444444444No Hit
GAGAACTTCAATACCCTTCTTCTTGAGCTTCTCAAGGAATGGAGAGTTCTC10.6944444444444444No Hit
GTTTTCAAAGTCCAAAGAGCGCAGACTAGCCACGGAGCTTGGATACGGCTG10.6944444444444444No Hit
CACTTGAGCCCTGATACATTTTCGGTGCCATGGAGCTAGACCAGTGAGCTA10.6944444444444444No Hit
CTGTTGAGCCCTGGTCAGCCTCATGGCTCATGCCGTAGGTGTGGGCCCGAT10.6944444444444444No Hit
CCATAACATTGTTTAAAGGATAACATCTCTGAGATAAGTAGCAACAGATAA10.6944444444444444No Hit
TTCCAGGACTTATTAGAACACTGAAGATGATGGGAACCCCTGTCTCTTATA10.6944444444444444No Hit
GGTGTAGGATAAGTGGGAGCTTCGGCGCAAGTGAAATACCACTACTTTTAA10.6944444444444444No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA10.6944444444444444No Hit
CCCAAGAGCTCTTATCGACGGAGTCGTTTGGCACCTCGATGTCGACTCATC10.6944444444444444No Hit
GTGGTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACAATCT10.6944444444444444No Hit
TACTTAGGCTTAGAGGGTGGTCCCCCTTTCTCGCGTAAAAGCGATCAGAAT10.6944444444444444No Hit
GACTAATGTAGGTAAGGGAAGTCGGCAAAATCGATCCGTAACTTCTGGATA10.6944444444444444No Hit
ATTCTCAAACTTTAAATAGGTAGGACGTGTCGGCTGCTTTGTTGAGCCGTC10.6944444444444444No Hit
NTATCAACGCAGAGTACGGGGAGAACTAGCTATCTACAGGTTCGGTTAGCT10.6944444444444444No Hit
GTATGTGACATTAATCCTAACATGTTAAACGTTGGGAAACAACGAGCTGCT10.6944444444444444No Hit
GCCCAGATCGTACGCTAAGGTCCCTAAGCAATCACTTAGTGGAAAAGGAAG10.6944444444444444No Hit
ACGTAGGAGGCTGCGATAAGCCTCGGGGAGCTGTCAACCGAGCTGTGATCC10.6944444444444444No Hit
GTTACACACTCCTTAGCGGATTTCGACTTCCATGACCACCGTCCTGCTGTC10.6944444444444444No Hit
GTATCGAGCCAGTCGTCAATATCATCCACTTCACGTAGGCTAAAAGGCACT10.6944444444444444No Hit
CATTGCATGTTTCCTCTGATCCTGTGTTGCTTCGTTCAAAGTCCTTGTCTC10.6944444444444444No Hit
GCTAGGATCTTACGGGTTAAACGATGAGATAAATCTGTCTCTTATACACAT10.6944444444444444No Hit
GTACGGGATCTTCTTCGCAAATCTCAACAATGGCTGTCTCTTATACACATC10.6944444444444444No Hit
GTACGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC10.6944444444444444No Hit
GAGAATGTTCTGGTTCGAGATGATGGTCACATAACTGTCTCTTATACACAT10.6944444444444444No Hit
TGAGTCACCACCCGGTATTCAGGAATAACAGCTGTATAACTGTCTCTTATA10.6944444444444444No Hit
TTCCTGCACCGATCATGTGGAGTGATGGAGGGACGCATTACGCTATCCAAT10.6944444444444444No Hit
GTGTATGGTCTAAAACTCATACAAATGTCGATAGTGTTACAAAACAAGGAC10.6944444444444444No Hit
GTTTCTTATTCCTGCATTCTAATTTGAATGCATTGTAATCTGAGAGACTGT10.6944444444444444No Hit
ACTTGGAGCTCTCGATTCCGTGTGACGGCTCAACAAAGCAGCCGACACGTC10.6944444444444444No Hit
TTGTCGCATTGAACCACAATTGATTGTAGTTTTGAGTTATATCGAAATCCT10.6944444444444444No Hit
CGTTAATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATAC10.6944444444444444No Hit
TCCGTATAGATATCATCATCTACATCCAGAAAGGCCGTATGCTTTGGAAGA10.6944444444444444No Hit
CCCCCAATGTTGTTCTTATCATGAGCATCGGTTTCATTGCTGTCTCTTATA10.6944444444444444No Hit
CCTATGATGTTATCCCATGCTAATGTATCCAGCTGTCTCTTATACACATCT10.6944444444444444No Hit
TGAGAAATTCATGTATTGAGCGTACCGATGAAACGACCCAAAACAAGTCCT10.6944444444444444No Hit
TTAAGCTTACTGATAACGATTACCAATCACCGAATACATTGAAGTTGCTTG10.6944444444444444No Hit
GCGTAATAGCTCACTGGTCTAACAGAGACCCCGCGCCGAAGATGTAACGGG10.6944444444444444No Hit
GTACAGGAGTAACTCTATCAAATGCATCACTAGATATTGCATTCCACGATA10.6944444444444444No Hit
GAACTGAGATATCGTTTAAGCAAAGGCATCAATCAAATCATTCAATTGCTC10.6944444444444444No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT10.6944444444444444No Hit
GGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCAC10.6944444444444444No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTGTTGAAACCCCCCTG10.6944444444444444No Hit
GGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGCGCGCTTAAGCGCCA10.6944444444444444No Hit
GCATCATTCAAATTTCTGCCCTATCAACTTTCAATGGTAGGATAGTGGCCT10.6944444444444444No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA10.6944444444444444No Hit
ATCACCTTCTTTGGCCAGATCTTCCAACCTTTTCACAATTACAGTTCACAG10.6944444444444444No Hit
CTGTAGTTCTCTATATCACTAAAACTCGGATAGAGAATCAACAGTCGTTGT10.6944444444444444No Hit
AGCTTATGCTCTGACCCGAGTAGCATGGGGCACGTGGAATCCCGTGTGAAT10.6944444444444444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTAAAAAACTTTC10.6944444444444444No Hit
ATCTTATCATTCGATGATAAAGACATTTCCACCGAATACTCTGCGCTTATG10.6944444444444444No Hit
ATTACAAGTAGTGTGTATGATGAGATTAGTGGTTTGTTGGTGTGAGTTACT10.6944444444444444No Hit
AGATTACTGTAGACTTGGAAGACTATGGGTGCAGAGAGAGGACATTTGAGT10.6944444444444444No Hit
GAAGAGTCAGTTTGTTATTTTTGAGGGCCAAGCTGCTAAGCTGTCTCTTAT10.6944444444444444No Hit
TTGTTACCATGGGAGATGAACCTTATCCAGACATATCACATCCTGTCTCTT10.6944444444444444No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCGC10.6944444444444444No Hit
CTCGTGTACAGTTCTTCGCTGAAACAGTGTAACTATTTAGCCTGTCTCTTA10.6944444444444444No Hit
GGGAAGCTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA10.6944444444444444No Hit
GCGGTGCATGGTTTGGAAGACGCCAACCGAATCGATGACGTCGAGGTTGAT10.6944444444444444No Hit
TAGCCCTGTAGATTCGTTCCTATGGTTCGATCCTTCCCAGTAAAACGCGGC10.6944444444444444No Hit
GTACAAACGAGAACGGCCTGTATTCGGAGTTTTCCTGGGGTTGGTAAGGCG10.6944444444444444No Hit
CTTTTTTCCTTTCCCTCACGGTACTTGTACGCTATCGGTCATTGAGGAATA10.6944444444444444No Hit
CGTGATTTACATATTGTGTCCTATAGCGCAAGATGTAGCGTCGGTTTTAAA10.6944444444444444No Hit
CTCAAGTGATTCACCGAAGCGACGAGACCTTGAAAGCTGCTTTTTCAAGTG10.6944444444444444No Hit
GAATACATGGTGGATGATACAATACAAATACATTGCACTGTCTCTTATACA10.6944444444444444No Hit
TCGTCATCGTCGGCTGGCTTGTTGCGATTGCCTGCATCTTTGGCGTGTTCA10.6944444444444444No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA10.6944444444444444No Hit
GTTCGGATTGTTCTCTGCAACTCGGGAACATGAAGTTGGAATCGCTAGTAA10.6944444444444444No Hit
AGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAG10.6944444444444444No Hit
GCTTCACAGTCTCTTGAGCTCTCCTTCATCATCGCCGAACGTCTTAGAAAG10.6944444444444444No Hit
GAATGGAAGCCCCGGTAAACGGCGGCAGTAACTCTAACTGCTGTCTCTTAT10.6944444444444444No Hit
CCTTTTGCATGATGGGTCAGCGAGGAAATGGGAAGAGCGGCTTAAGCCATT10.6944444444444444No Hit
TCCGAGCACGAACAGGGTTGCACAAGCCAGCCCGGCTCGCGAACCTGTCAA10.6944444444444444No Hit
GTACACATGGAGGCTGCACGATAAGCCCTTCGAATTACATAGCTCACGAGG10.6944444444444444No Hit
CAACACCATGCTCAATAGGATACTTCAAGGTAAGAATACCTCTCTTGGATC10.6944444444444444No Hit
GTATCAACGCAGAGTACGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTG10.6944444444444444No Hit
TAATTTGATGCGTTAAGAAATATATGTACAGGTTACGGTTTCTAAAGAGTA10.6944444444444444No Hit
CTTCTTGACTATGACAAGAGTCGCGACTATAAACAGACTCGCGACTATGGC10.6944444444444444No Hit
AATCAGAAGATGGGATGAGATTCTTGTTGCTTTGTAGAGTTCTACTTGGAC10.6944444444444444No Hit
TAACATTACTCATCATATTCATATCATCGTAATATCCGTCGATCCCAACCG10.6944444444444444No Hit
CGAGAGACCGATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTT10.6944444444444444No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTAAAAAAAAAA10.6944444444444444No Hit
CCTTTTCAGCCACTTTTTGCTCGACCTTTGTCTGCTGCTTCACAGCTTGGC10.6944444444444444No Hit
TCCTGGTGGTGTAAGGTCCTTGAAAGGGTCTTCGAGAGCAAATGTTGCGGT10.6944444444444444No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCTTTGAACA10.6944444444444444No Hit
ATGTACCATTTGGACGGAAAACATACGCACCCGAGTTTTGAGGTATAAGAG10.6944444444444444No Hit
GTTAAAGATAGAGAAAGAGAAAACAGCTCACGTAACCAAACACTCCACTTC10.6944444444444444No Hit
GGCTAAACCGTGTGCATGTGATACCTGGTAGGGGTTGTGTGTGCGGTGTTG10.6944444444444444No Hit
CCTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCAGTTACCCCTAC10.6944444444444444No Hit
GGTCTAAGTCGCCGGCTCATGCTTCAATAGGCACGCCATAACACACGTAAG10.6944444444444444No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC10.6944444444444444TruSeq Adapter, Index 12 (95% over 21bp)
CTATCTGTAGCGTTTGCTGGGAACAGTGACAAGGCTCTGAGATACGTTCTT10.6944444444444444No Hit
CATCATGAACAGTGCTCTTGTCCATCTTAGCATCACGAAGACACTTCTCAA10.6944444444444444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCT10.6944444444444444No Hit
GAGCAGAGCGTACCGCCCTGCCATAGTCGCGAGTCTGTTTATAGTCGCGAC10.6944444444444444No Hit
GGTATCAACACAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.6944444444444444No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC10.6944444444444444No Hit
GTGCGGTGCTCTTCCAGCCGCTGGACCCTACCTCCGGCTGAGCCGTTTCCA10.6944444444444444No Hit
TTCTAGTTCTTCCTATTTGACCCGAAACCGATCGATCTAGCCATGAGCAGG10.6944444444444444No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6944444444444444No Hit
CACATGAGCACTACTCATGTACCTCTGCCTGAATAAAATCTGTCTCTTATA10.6944444444444444No Hit
ATCAAATGATTTGGCTTTTATTAAAGCTTGAAAAGAGAACTTAATCTTCTT10.6944444444444444No Hit
GACTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGTCTTT10.6944444444444444No Hit
GGTACGAAGTAACTCGACTGAAAGAAGAGGGGCTTGCTGGTTTTTCCATCA10.6944444444444444No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers