##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23V3BCXX l02n01 kb2_2_1.34100000009f12.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27196 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.94388880717753 38.0 38.0 38.0 32.0 38.0 2 36.956905427268715 38.0 38.0 38.0 32.0 38.0 3 36.87887924694808 38.0 38.0 38.0 32.0 38.0 4 36.95061773790263 38.0 38.0 38.0 32.0 38.0 5 36.93565230180909 38.0 38.0 38.0 32.0 38.0 6 38.93098249742609 40.0 38.0 40.0 38.0 40.0 7 38.84416826003824 40.0 38.0 40.0 38.0 40.0 8 38.842881306074425 40.0 38.0 40.0 38.0 40.0 9 38.8272907780556 40.0 38.0 40.0 38.0 40.0 10 38.78908663038682 40.0 38.0 40.0 38.0 40.0 11 38.814016767171644 40.0 38.0 40.0 38.0 40.0 12 38.793278423297544 40.0 38.0 40.0 38.0 40.0 13 38.816333284306516 40.0 38.0 40.0 38.0 40.0 14 38.78724812472422 40.0 38.0 40.0 38.0 40.0 15 38.75853066627445 40.0 38.0 40.0 38.0 40.0 16 38.77776143550522 40.0 38.0 40.0 38.0 40.0 17 38.7566553904986 40.0 38.0 40.0 38.0 40.0 18 38.78768936608325 40.0 38.0 40.0 38.0 40.0 19 38.826114134431535 40.0 38.0 40.0 38.0 40.0 20 38.824385939108694 40.0 38.0 40.0 38.0 40.0 21 38.82490072069422 40.0 38.0 40.0 38.0 40.0 22 38.8037946756876 40.0 38.0 40.0 38.0 40.0 23 38.79695543462274 40.0 38.0 40.0 38.0 40.0 24 38.83736578908663 40.0 38.0 40.0 38.0 40.0 25 38.833982938667454 40.0 38.0 40.0 38.0 40.0 26 38.765259596999556 40.0 38.0 40.0 38.0 40.0 27 38.74205765553758 40.0 38.0 40.0 38.0 40.0 28 38.66267098102662 40.0 38.0 40.0 38.0 40.0 29 38.51478158552728 40.0 38.0 40.0 38.0 40.0 30 38.482497426092074 40.0 38.0 40.0 38.0 40.0 31 38.40700838358582 40.0 38.0 40.0 38.0 40.0 32 38.23227680541256 40.0 38.0 40.0 38.0 40.0 33 38.082769524930136 40.0 38.0 40.0 38.0 40.0 34 37.892778349757315 40.0 38.0 40.0 32.0 40.0 35 37.68925577290778 40.0 38.0 40.0 32.0 40.0 36 37.487424621267834 40.0 38.0 40.0 32.0 40.0 37 37.38950580967789 40.0 38.0 40.0 32.0 40.0 38 37.28802029710251 40.0 38.0 40.0 32.0 40.0 39 37.07449624944845 40.0 38.0 40.0 32.0 40.0 40 36.87060597146639 40.0 38.0 40.0 27.0 40.0 41 36.695396381820856 40.0 38.0 40.0 27.0 40.0 42 36.44774966906898 40.0 38.0 40.0 27.0 40.0 43 36.13362259155758 40.0 38.0 40.0 27.0 40.0 44 35.910648624797766 40.0 38.0 40.0 13.0 40.0 45 35.60027209883806 40.0 38.0 40.0 13.0 40.0 46 35.29423444624209 40.0 38.0 40.0 13.0 40.0 47 34.897448154140314 40.0 38.0 40.0 13.0 40.0 48 34.472569495514044 40.0 38.0 40.0 13.0 40.0 49 34.17329754375643 40.0 32.0 40.0 13.0 40.0 50 33.839608765995 40.0 32.0 40.0 13.0 40.0 51 33.267759964700694 38.0 27.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.9889280067245849 2201 2 0.04886296675530133 2201 3 0.3169424311074991 2201 4 0.8268362721539049 2201 5 0.37931598755994855 2201 6 0.5933213499612862 2201 7 0.6727016528738261 2201 8 0.7024532155258072 2201 9 0.3301404327002615 2201 10 0.2808438842050194 2201 11 -0.24998630842756597 2201 12 0.7596487200333755 2201 13 0.5399102148677031 2201 14 0.5574321477863293 2201 15 0.5239138951723774 2201 16 0.2425946365636662 2201 17 0.5744849010534523 2201 18 0.7692288540702137 2201 19 0.5942875109537198 2201 20 0.7867986479239164 2201 21 0.45240342754726015 2201 22 0.46248232543573664 2201 23 0.4882580891794177 2201 24 0.7986865689570806 2201 25 0.6232009151013358 2201 26 0.1833401164284041 2201 27 0.11852366694800764 2201 28 0.8168147201536229 2201 29 0.9526421638866012 2201 30 0.8579153986648791 2201 31 0.6800666126798305 2201 32 -0.5436989718913168 2201 33 -1.0076295274918365 2201 34 -0.34011835821529957 2201 35 0.15571717898784954 2201 36 0.27625687128495713 2201 37 0.11270910433620429 2201 38 0.31646583015365337 2201 39 0.42453891059570026 2201 40 -0.11999432640107699 2201 41 0.1041410496390398 2201 42 1.1523334988455503 2201 43 1.732704165884357 2201 44 0.026398725085073238 2201 45 -0.6104620885121221 2201 46 0.7306450079685618 2201 47 1.3127594952590798 2201 48 0.450247014297382 2201 49 0.32574607218459306 2201 50 0.4148472048885097 2201 51 -1.0820127224462013 1101 1 -0.06512604732947125 1101 2 -0.34843433054199835 1101 3 -0.176300812135743 1101 4 0.49034978566741927 1101 5 -0.10176509352113072 1101 6 -0.4661381094981749 1101 7 -0.4908118606396883 1101 8 0.017318080390673174 1101 9 0.2132485408083653 1101 10 -0.4036155752544417 1101 11 0.6135272050859513 1101 12 -0.42683776645311156 1101 13 -0.8019816770241945 1101 14 -0.1398651495109675 1101 15 -1.3625725913141054 1101 16 -0.5330810391120124 1101 17 -0.31200158543303047 1101 18 -1.1591495243081624 1101 19 -0.5624692458030367 1101 20 -0.18347162234636016 1101 21 0.37267369781753246 1101 22 3.2016327357808905E-4 1101 23 0.02204187296320015 1101 24 -0.11752964725913984 1101 25 0.20022794212835748 1101 26 0.5056374137257009 1101 27 0.3496047480290869 1101 28 -0.250752847413942 1101 29 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0.4128327403925667 1213 10 -0.27107919271806225 1213 11 -0.5538324622737179 1213 12 -0.6134282030435472 1213 13 -0.14085901590153327 1213 14 -0.28487554452136266 1213 15 0.2969908182492986 1213 16 0.3349023288713582 1213 17 0.001407977976533914 1213 18 -0.5307711459297835 1213 19 0.001979818646027809 1213 20 0.22141403253930036 1213 21 -0.32067349552966107 1213 22 -0.8759792130258006 1213 23 -0.3963572954359691 1213 24 -1.1513134310429223 1213 25 -0.8075683156679005 1213 26 -0.15704449895621053 1213 27 0.3069852054095463 1213 28 0.4129685663074696 1213 29 -0.35505014380570543 1213 30 -0.14400767825819827 1213 31 0.4531435357567517 1213 32 0.31591641272407145 1213 33 0.27121662635431676 1213 34 0.00795856486161739 1213 35 0.27494794821861746 1213 36 -0.5948969748688882 1213 37 -0.30844474181764525 1213 38 -0.27776493907711597 1213 39 -0.6369995509427611 1213 40 0.19923644282968667 1213 41 -0.057397411899422934 1213 42 -0.10535880884675919 1213 43 -0.3615266033464124 1213 44 0.378321802008152 1213 45 0.060691757641727406 1213 46 0.08833731566086556 1213 47 0.6550671875667717 1213 48 2.027170091220462 1213 49 2.0949768414153596 1213 50 1.9013856664269682 1213 51 0.8487565083230351 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 2.0 15 0.0 16 3.0 17 4.0 18 3.0 19 3.0 20 1.0 21 5.0 22 12.0 23 14.0 24 36.0 25 75.0 26 115.0 27 175.0 28 251.0 29 364.0 30 485.0 31 629.0 32 742.0 33 880.0 34 1072.0 35 1213.0 36 1400.0 37 1991.0 38 3164.0 39 14557.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.527869818128266 16.434724983432737 12.760474191885724 19.276931006553273 2 17.12016473010737 22.488601264891898 39.66392116487719 20.727312840123545 3 22.253272540079426 30.254449183703485 26.088395352257688 21.403882923959404 4 13.373290189733783 16.222973966759817 34.6999558758641 35.703779967642305 5 17.925430210325047 35.12281217826151 27.996764230033826 18.954993381379616 6 29.842623915281656 35.836152375349315 14.722753346080305 19.59847036328872 7 28.68436534784527 31.567142226798055 20.6170025003677 19.13148992498897 8 29.386674510957494 26.59214590380938 18.653478452713635 25.36770113251949 9 29.496984850713343 15.296367112810708 21.440653037211355 33.7659949992646 10 22.87836446536255 24.727901161935577 25.051478158552726 27.342256214149142 11 32.03412266509781 25.34563906456832 23.415208118841004 19.205030151492867 12 29.28371819385204 24.62494484483012 27.2282688630681 18.863068098249745 13 29.923518164435947 23.051183997646714 27.294455066921607 19.730842770995736 14 24.768348286512722 20.547139285188997 30.86851007501103 23.81600235328725 15 22.22753346080306 25.86777467274599 29.559494043241653 22.345197823209297 16 19.918370348580673 29.58523312251802 27.63641712016473 22.859979408736578 17 20.488307103985882 24.73525518458597 28.018826297985 26.757611413443154 18 24.672745992057653 22.654066774525667 31.600235328724814 21.072951904691866 19 26.76128842476835 22.19076334755111 32.037799676423 19.01014855125754 20 24.191057508457124 21.275187527577586 32.203265186056775 22.330489777908515 21 24.161641417855567 23.08060008824827 32.89454331519341 19.86321517870275 22 21.117076040594203 24.81247242241506 33.48286512722459 20.587586409766143 23 21.598764524194735 26.040594205030153 33.872628327695246 18.488012943079866 24 21.209001323724078 26.375202235622886 33.54169730842771 18.87409913222533 25 19.006471539932342 27.842329754375644 34.56758346815708 18.583615237534932 26 18.54316811295779 28.702750404471246 33.93146050889837 18.8226209736726 27 18.40711869392558 28.76158258567436 33.898367406971616 18.932931313428444 28 18.46962788645389 28.72113546109722 32.26945138991028 20.539785262538608 29 18.995440505956758 29.541108986615676 31.427415796440656 20.03603471098691 30 18.285777320194146 29.54846300926607 31.84659508751287 20.319164583026918 31 18.837329018973378 28.555669951463454 31.537726136196497 21.06927489336667 32 19.41094278570378 28.19899985291955 32.265774378585085 20.124282982791584 33 19.469774966906897 28.92337108398294 32.24371231063392 19.363141638476247 34 18.93660832475364 30.743491689954404 30.98985144874246 19.330048536549494 35 18.49904397705545 31.155316958376233 30.901603176937787 19.444035887630534 36 17.962200323576997 30.967789380791295 32.177526106780405 18.892484188851302 37 18.175466980438298 31.717899691131045 30.798646859832328 19.307986468598322 38 17.69377849683777 31.103838799823503 32.328283571113396 18.87409913222533 39 17.85924400647154 31.1075158111487 31.111192822473893 19.922047359905868 40 17.598176202382703 31.820856008236504 31.2509192528313 19.330048536549494 41 18.13869686718635 31.217826150904543 30.335343432857776 20.30813354905133 42 18.25636122959259 31.276658332107665 29.61832622444477 20.848654213854978 43 17.134872775408148 30.754522723929988 29.945580232387115 22.165024268274745 44 16.605383144580085 31.298720400058833 29.614649213119577 22.481247242241505 45 17.065009560229445 30.074275628768937 29.74702162082659 23.113693190175024 46 17.56508310045595 29.55214002059126 29.522723929989702 23.360052948963084 47 16.873804971319313 29.386674510957494 29.11089866156788 24.628621856155316 48 16.678923371083982 29.213854978673336 28.35343432857773 25.75378732166495 49 17.502573907927637 28.938079129283718 28.070304456537727 25.489042506250918 50 17.11281070745698 28.50051478158553 28.04824238858656 26.338432122370936 51 17.05397852625386 28.324018237976173 27.540814825709663 27.081188410060303 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 57.0 1 33.0 2 9.0 3 13.5 4 18.0 5 17.0 6 16.0 7 20.5 8 25.0 9 34.5 10 44.0 11 101.0 12 158.0 13 330.5 14 503.0 15 681.5 16 860.0 17 894.5 18 929.0 19 926.0 20 923.0 21 882.5 22 842.0 23 965.0 24 1088.0 25 1078.5 26 845.0 27 621.0 28 541.0 29 461.0 30 442.5 31 424.0 32 536.0 33 648.0 34 698.5 35 749.0 36 660.0 37 571.0 38 506.0 39 441.0 40 484.5 41 528.0 42 649.0 43 770.0 44 855.5 45 941.0 46 1104.0 47 1267.0 48 1425.0 49 1583.0 50 1624.5 51 1666.0 52 1714.5 53 1763.0 54 1715.0 55 1667.0 56 1552.0 57 1437.0 58 1309.5 59 1182.0 60 1066.5 61 951.0 62 853.0 63 755.0 64 670.0 65 585.0 66 533.0 67 481.0 68 426.5 69 372.0 70 333.0 71 294.0 72 254.5 73 215.0 74 175.0 75 97.5 76 60.0 77 52.0 78 44.0 79 33.5 80 23.0 81 19.0 82 15.0 83 10.0 84 5.0 85 3.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12501838505662596 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 27196.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.62438270854724 #Duplication Level Percentage of deduplicated Percentage of total 1 92.113781579518 71.50275434060035 2 4.71242411158334 7.31598025445062 3 1.3339666273698236 3.1064500801015567 4 0.6621101349575614 2.0558356204461448 5 0.35370958900414345 1.3728244252270294 6 0.21547473620902566 1.00356560325076 7 0.09985635742225396 0.5425901673098567 8 0.058895506158874546 0.3657381847912063 9 0.06796639166783651 0.4748264278328842 >10 0.300878263183651 4.32480285980105 >50 0.038087860201521474 1.9919732965364134 >100 0.04284884272396271 5.942658739652173 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 295 1.08471834093249 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 238 0.8751286953963818 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 213 0.7832034122665098 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 178 0.654508015884689 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 161 0.591998823356376 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 141 0.5184585968524783 RNA PCR Primer, Index 27 (95% over 21bp) TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 139 0.5111045742020885 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 128 0.4706574496249448 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 115 0.4228563023974114 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 96 0.3529930872187086 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 87 0.31989998529195474 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 86 0.31622297396675986 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 62 0.22797470216208265 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 53 0.19488160023532872 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 52 0.19120458891013384 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52 0.19120458891013384 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51 0.18752757758493896 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45 0.1654655096337697 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 43 0.15811148698337993 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.1397264303574055 No Hit GCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.1397264303574055 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 35 0.12869539638182087 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 30 0.11031033975584645 No Hit GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 29 0.10663332843065157 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 29 0.10663332843065157 No Hit GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.10295631710545668 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28 0.10295631710545668 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0036770113251948814 17 0.0 0.0 0.0036770113251948814 18 0.0 0.0 0.0036770113251948814 19 0.0 0.0 0.0036770113251948814 20 0.0 0.0 0.0036770113251948814 21 0.0 0.0 0.0036770113251948814 22 0.0 0.0 0.011031033975584645 23 0.0 0.0 0.02206206795116929 24 0.0 0.0 0.04412413590233858 25 0.0 0.0 0.07721723782909251 26 0.0 0.0 0.10663332843065157 27 0.0 0.0 0.16178849830857478 28 0.0 0.0 0.3198999852919547 29 0.0 0.0 0.6103838799823503 30 0.0 0.0 1.0442712163553465 31 0.0 0.0 1.584791881158994 32 0.0 0.0 2.1216355346374467 33 0.0 0.0 2.7173113693190176 34 0.0 0.0 3.2063538755699366 35 0.0 0.0 3.699073393146051 36 0.0 0.0 4.298426239152817 37 0.0 0.0 5.103691719370496 38 0.0 0.0 6.162670981026621 39 0.0 0.0 7.302544491837035 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCAG 460 0.0 20.054346 6 CGCAGAG 485 0.0 19.020618 8 ACGCAGA 495 0.0 18.636366 7 CAGAGTA 500 0.0 18.449999 10 AGAGTAC 500 0.0 18.449999 11 GCAGAGT 505 0.0 18.267324 9 CGGGGTG 180 6.0665116E-8 15.0 4 GAGTACT 360 0.0 15.0 12 AGTACTT 370 0.0 14.594594 13 GGGGTGG 185 8.489769E-8 14.594594 5 ACGGGGT 195 1.6168451E-7 13.846153 3 GAGTACG 285 4.1836756E-11 13.421053 1 GTATCAA 725 0.0 13.344829 1 AGTACGG 290 5.638867E-11 13.189654 2 GTACTTT 435 0.0 12.931035 14 TACTTTT 435 0.0 12.931035 15 TACGGGG 255 2.4008841E-8 12.3529415 2 GTACGGG 385 0.0 12.272728 1 ACTTTTT 445 0.0 12.134831 16 TATCAAC 805 0.0 12.018633 2 >>END_MODULE