FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_96.34100000009f05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_96.34100000009f05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328595
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC3450.10499246793164838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAG507.143936E-526.99954237
GAGCCCG450.001224811424.99957741
CCTCGAT1001.3369572E-924.7495833
TGAGTAG1101.5097612E-1024.54503839
GCCAGTT702.6957623E-522.4996240
CGTATGC602.4625915E-422.49961941
GCGCCTA602.4625915E-422.49961941
GCCTCGA1104.0272425E-922.49961932
GCGAGCC500.00226141822.49961939
CGTCGGG1056.0756065E-821.4282114
ACACCGT855.014912E-621.1761136
TCCCGCA754.590394E-520.99964731
ACCTATA1301.20599E-920.7846971
GTTGTAT654.2081598E-420.7846971
TAGAGTG654.2298427E-420.768885
GGTCTGC654.2298427E-420.7688829
AGCCAGG1201.0981239E-820.62465126
CGTGGCG550.00393103420.45419939
CGGAACC550.00393103420.45419928
AGTCCGT550.00393103420.4541996
Produced by FastQC (version 0.11.2)