FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_95.34100000009ef7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_95.34100000009ef7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363508
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC12500.3438713865994696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.11334000902318518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1850.036.48559645
CGTCGGG250.002105469835.99912314
ATGCCGT1950.033.4607244
GTATTAA855.456968E-1231.798921
CGTATGC2100.031.07066741
TTCGCGC300.005143298329.99926810
GTTGCGA300.005143298329.99926831
TATGCCG2200.029.65836543
CCCACGA2200.029.65836519
GCCCACG2350.028.72270218
GTATGCC2300.028.36887242
CGAGACA2200.027.6129623
TCGTATG2450.027.55034840
ACGAGAC2400.027.18683622
TATCTCG2550.026.46994236
CACGAGA2500.026.09936321
TCTCGTA2500.026.09936338
ATCTCGT2550.025.5876137
CTCGTAT2600.025.09554139
TAGGGAG551.3659176E-424.5448555
Produced by FastQC (version 0.11.2)