FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_94.34100000009eea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_94.34100000009eea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474493
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC5740.12097122612978485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.10158210974661375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA1004.5474735E-1126.99892619
ACGAGAC1200.026.24895522
ATGCCGT1052.3592293E-923.5704944
CACGAGA1353.6379788E-1223.33240321
GCTGACG500.002263188422.49910533
GCCCACG1204.4929038E-1022.49910418
TATGCCG1204.4929038E-1022.49910443
GACTTGC1305.0931703E-1122.49910426
GTATGCC1104.0399755E-922.49910442
AATCTCG1104.0399755E-922.49910436
GTATCCG602.4652114E-422.49910431
CCACGAG1305.0931703E-1122.49910420
TACACCG702.6995043E-522.4991045
CGTATGC1156.7466317E-921.52088241
CCGACTA654.234316E-420.7684029
AGGGCGG654.234316E-420.76840227
ATCTCGT1201.10158E-820.62417837
TCTCGTA1201.10158E-820.62417838
GCGACTA550.00393409120.45373216
CGAGACT1452.1827873E-1020.17161223
Produced by FastQC (version 0.11.2)