FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_93.34100000009edd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_93.34100000009edd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088663
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC14100.12951666401815803No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA11550.10609343754678904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2050.032.92518218
TATGCCG2050.031.82913843
ATGCCGT2150.030.34871544
CGTATGC2400.029.06238241
CGAGACG2250.027.99859823
GTATGCC2450.027.55090942
CGCTATA2400.027.1861431
CCCACGA2450.026.6313219
CGCGGTA709.307114E-725.71418241
CGACGCT2500.025.19873828
ACGACGC2500.025.19873827
TGCCGTC2600.025.09605245
CCGAGCC2600.025.09489614
CCACGAG2600.025.09489620
GACGCTA2550.024.70464929
CGAGCCC2650.024.6214115
CCTAGTC551.3686027E-424.5442263
ACGCTAT2750.022.90794630
AGACGAC2850.022.8935925
CTCCGAG2800.022.49887712
Produced by FastQC (version 0.11.2)