FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_92.34100000009ec0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_92.34100000009ec0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781572
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC16720.21392782750661488No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA10660.1363917847619925No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT9940.12717958166362153No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA8640.1105464371804517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2250.032.99859245
GCCCACG2300.031.30300918
GTATGCC2300.031.30300942
ATGCCGT2300.031.30300944
GGCGAAT2350.030.6369932
CAAGGCG2300.030.32479129
CGTATGC2500.028.79877341
TATGCCG2500.028.79877343
GCGAATC2500.027.8988133
CGAGACC2700.026.6655323
CCACGAG2700.026.6655320
CCGAGCC2800.025.71318814
AGGCGAA2750.025.36255631
CCCACGA2900.024.82652719
CGAGCCC2950.024.40573915
TCGTATG3000.023.99897640
ACGGGCA952.1142114E-823.68323
ACGAGAC3050.023.6055522
CGTTACT1152.6557245E-1023.4772572
CCAAGGC3000.023.24900828
Produced by FastQC (version 0.11.2)