FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_91.34100000009eb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_91.34100000009eb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976837
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC25620.2622750776229811No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3600.041.24878345
CGAGACG3850.037.98589323
CCCACGA3950.037.59382619
TATGCCG4000.037.123943
CCGAGCC4000.037.123914
ATGCCGT4000.037.123944
GCCCACG4050.036.6655818
CCACGAG4100.036.2184420
CGTCGTA250.002107259835.99893645
CGAGCCC4300.035.05710215
ACGAGAC4250.034.94014422
AGCCCAC4350.034.1369217
CACGAGA4350.034.1369221
GACGATA4400.033.2376526
CGTATGC4500.032.99902341
AGACGAT4500.032.99902325
GAGCCCA4650.032.41839616
TCCGAGC4600.032.28165413
CTCCGAG4700.032.0735212
GTATGCC4700.032.0735242
Produced by FastQC (version 0.11.2)