FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_9.341000000098e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_9.341000000098e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482914
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG5390.11161407621232766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA708.112693E-1032.14211731
TAGTTCG406.161753E-428.1243534
TATGCCG856.4628694E-926.4699843
CTGCGCG651.5216463E-524.2302119
GGGGCAA1104.0381565E-922.49948318
CGCGCTA602.4650196E-422.49948312
GCCCACG1003.634159E-822.49948118
TCGGCAG500.00226302622.49948134
GTATGCC1003.634159E-822.49948142
CGAGACG1003.634159E-822.49948123
ACGCTAT1156.7466317E-921.52124430
ATGCCGT1056.090886E-821.4280844
GCGCGCT1700.021.17598311
CGGGGCA1501.4551915E-1120.99951717
TCGGGCG1301.2223609E-920.76875137
CGTATGC1109.9564204E-820.45407541
CTCGTAT1109.9564204E-820.45407539
CGGCAGG550.003933807420.45407535
GCTATAT1109.9564204E-820.45407532
CTAGTGT550.003933807420.4540754
Produced by FastQC (version 0.11.2)