FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_89.34100000009e9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_89.34100000009e9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767894
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTTAGACTCCCATGTAATAAGAGAATCTTGCATCCATCTTGGTTTTGAA9400.12241272884017847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTCCC2100.024.6419647
CTTAGAC3550.020.9147363
GTATCAA8800.020.4871581
TATACTA807.560928E-519.6867875
ATGCCGT706.982282E-419.28501544
TTAGACT3650.019.1088964
CCCATGT2750.018.81750111
GTCTAGG600.006448984618.7798921
CGGGGGG600.00650993518.74932117
CGAGCCC600.00650993518.74932115
ATTATAC1253.8305734E-717.9993483
GTCCTAC1902.3646862E-1117.791481
ATAGTTA1305.778602E-717.3070662
AGACTCC3850.016.947446
CCATGTA3100.016.69294412
CTCCCAT3250.016.6147829
ATATAGG952.783789E-416.605381
TCTAGAC1501.4215038E-716.4994033
TAGACTC4100.016.4628185
ACTCCCA3300.016.3630458
Produced by FastQC (version 0.11.2)