FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_88.34100000009e8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_88.34100000009e8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC110.9945750452079566No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC110.9945750452079566No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC30.27124773960216997No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC30.27124773960216997No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC30.27124773960216997No Hit
CTCAGTACACTTCTTTTCATACCTCAAGAGACTAAAACTAGTTTGAGGAGA20.18083182640144665No Hit
GTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGCGGATGGGGGCCGGCGAT20.18083182640144665No Hit
TTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTT20.18083182640144665No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT20.18083182640144665No Hit
GCTATGAACATAGTGGAGTATGTGTCTTTCTTACTAGTTGGAACATCTTCT20.18083182640144665No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)