FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_87.34100000009e70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_87.34100000009e70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388903
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC606.4101187E-933.74903541
GTATGCC606.4101187E-933.74903542
TCGTATG702.8767317E-828.92774440
GTACTAT406.113593E-428.1604021
TATGCCG755.6204954E-826.9992343
ATGCCGT856.4555934E-926.46983144
CTCGTAT801.05033905E-725.31177739
CCCACGA801.05033905E-725.31177719
TAGGATA450.001225299624.9992874
ATACCGT551.3661955E-424.5447526
GCCCACG851.8874744E-723.8228518
GTATCAA7000.023.8156551
GGTATCA2500.022.5283221
TTAGGAC500.002262312522.4993573
GGCGTTT500.002262312522.49935736
ATACTAT500.002262312522.4993576
TTTGCGA702.6976515E-522.49935710
TATCTCG955.513866E-721.3151836
CGAGACT955.513866E-721.3151823
ACCCCGC550.003932578520.453967
Produced by FastQC (version 0.11.2)