FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_86.34100000009e63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_86.34100000009e63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288468
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC3270.11335746079287824TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT300.00514152529.9993044
CGCCTAA300.00514152529.99930442
TATAATG300.00514152529.9993042
CGTATGC702.8710929E-828.92790241
CTCGTAT702.8710929E-828.92790239
TCGTATG702.8710929E-828.92790240
CCCACGA803.3742253E-928.12435219
ACGCGCG450.001224492824.99942222
CGAGACT651.5188873E-524.23021123
GCGGTAA851.8838546E-723.8229841
GGTATCA2400.023.4613591
GAGATAG500.002247222.5229051
GCCCCAA1003.621426E-822.4994830
GCCCACG903.2700154E-722.4994818
TATGCCG903.2700154E-722.4994843
GTATGCC903.2700154E-722.4994842
TAATGGT500.002260834222.499484
ATTACTC602.461684E-422.4994773
CCTAAAG602.461684E-422.4994773
GTATCAA6300.021.8078921
Produced by FastQC (version 0.11.2)