FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_84.34100000009e3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_84.34100000009e3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896496
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC13220.14746301154717925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG1500.035.99897418
TATGCCG1600.033.7490443
CCCACGA1650.032.72633719
ATGCCGT1650.032.72633744
GTATGCC1900.028.42024242
GTCGCGC1350.028.3325258
TCGCGCG1350.028.3325259
GCGCGCT1750.028.28490811
CGTATGC1950.027.69151741
CGCGCGC1400.027.32064810
TCGTATG2000.026.9992340
GGTCGCG1450.026.3785577
CGAGACC1800.024.99928723
GCGCTTT450.001226548124.99928713
GGGGCTA1450.024.82687836
CTCGTAT2100.024.64215539
ACGAGAC2400.023.4368322
AGCGCCA1550.023.22514520
CCGAGCC2450.022.9585314
GCGCCAT1600.022.4993621
Produced by FastQC (version 0.11.2)