FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_83.34100000009e20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_83.34100000009e20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759340
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC466276.14046408723365No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT131931.7374298733110334No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC101771.3402428424684594No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG41170.5421813680301315No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA41030.5403376616535412No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA35890.4726472989701583No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG30950.40759080253904706No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC29750.39178760502541676No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA19760.2602259857244449No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA17710.23322885663865986No Hit
AAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACGAG10530.1386730581821055No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG10400.1369610451181289No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC9570.12603050017120132No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC9450.12445018041983828No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA9260.12194800748018014No Hit
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC7700.10140385071246083No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA7680.10114046408723365No Hit
AAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC7610.10021861089893856No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCC207.03299E-444.99841717
TGCCGTC48050.044.15557545
ATGCCGT62950.033.77562344
TATGCCG67500.031.49889443
TATCTCG67600.031.35245136
CGTATGC67750.031.34945141
GTATGCC67850.031.3364142
ATCTCGT67450.031.322137
TCGTATG67900.031.28019740
CGAGACA68150.031.26449223
ACGAGAC68600.031.19059822
TCTCGTA67800.031.1604138
CTCGTAT67850.031.1374539
CACGAGA68750.031.12254321
AGACAAC69150.030.84490425
CAACATA69450.030.71166228
GACAACA69500.030.68957126
ATATCTC68900.030.66292835
ACAACAT70350.030.35074827
AACATAA70350.030.35074829
Produced by FastQC (version 0.11.2)