FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_82.34100000009e13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_82.34100000009e13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213859
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC373723.078776035766922No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA71020.5850761908920229No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT65110.5363884932269729No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA64380.530374615173591No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG48750.4016117193183063No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG47450.39090207346981815No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG45310.3732723487653838No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC45150.37195423850710835No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG43370.35729026188379376No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC41040.33809528124765725No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG39850.32829183620173347No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA36400.2998700837576687No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA32250.2656815989336488No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG32000.2636220516550934No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG28770.23701270081615738No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA27600.22737401955251804No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA27530.22679734631452253No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA26260.21633484613946102No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA24870.20488376327069288No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG23420.1929383890550715No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATAC23080.1901374047562361No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG22190.18280541644457882No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC18130.14935836864083885No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTAT17860.147134057579999No Hit
TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA17630.145239274083728No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA17440.1436740181520259No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTA16370.1348591557998087No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA16240.13378819121495988No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACA15870.13074006124269788No Hit
TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA15770.1299162423312757No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA15630.12876289585528466No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA14340.11813563189793873No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTAT14270.11755895865994322No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACA14150.11657037596623661No Hit
ATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG13970.1150875019256767No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCC13850.1140989192319701No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC13710.11294557275597908No Hit
GTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGAG13700.11286319086483686No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC13600.11203937195341468No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTC13380.11022697034828591No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC13380.11022697034828591No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAACTGTCTCTT13240.10907362387229488No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTA13220.10890886009001047No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTCC12970.10684931281145504No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTATA12960.10676693092031282No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC12690.10454261985947298No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC12340.10165925366949538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC39400.044.2559145
ATGCCGT45650.038.1474944
TATGCCG50700.034.3477943
GACTTGC51100.034.12295526
CGAGACT51350.034.0882723
ACGAGAC51350.034.0882722
TCGTATG51050.034.0682340
AGACTTG51350.034.04445325
ATCTCGT51000.034.01339737
CTCGTAT50900.033.99181739
GTATGCC51350.033.9568242
GCCGTCT401.5610494E-533.74870345
AATCTCG51350.033.73774736
GCTGGAA51450.033.71590431
CCACGAG51800.033.7052720
TTGCTGG51400.033.7049329
TCTCGTA51500.033.59579538
CACGAGA52050.033.58660521
GAGACTT52450.033.33046324
CTGGAAT51950.033.30478332
Produced by FastQC (version 0.11.2)