FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_81.34100000009e06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_81.34100000009e06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251782
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA11280.4480066088918191No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA10890.4325170186907722No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG10470.4158359215511832No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC9890.3928001207393698No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG9600.3812822203334631No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC8710.3459341811567149No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC8650.3435511672796308No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC7080.281195637495929No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT6280.2494221191348071No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT5780.2295636701591059No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG5320.21129389710146076No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG5130.20374768649069433No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT4950.1965986448594419No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT4740.1882580962896474No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA4670.18547791343304923No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTC4570.18150622363790897No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA4420.17554868894519862No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA4400.1747543509861706No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT4340.17237133710908645No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA4310.17117983017054436No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA4030.1600590987441517No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3980.15807325384658155No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT3980.15807325384658155No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT3750.14893836731775903No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA3730.14814402935873097No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA3570.1417893256865066No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG3550.14099498772747854No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCTC3510.13940631180942242No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG3360.13344877711671208No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC3200.1270940734444877No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC3170.12590256650594564No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC3090.12272521466983344No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACAT3080.12232804569031941No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTAT3050.12113653875177734No Hit
GCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA3050.12113653875177734No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG2960.11756201793615112No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC2720.10802996242781453No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG2670.10604411753024441No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCC2650.10524977957121638No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC2650.10524977957121638No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAT253.883012E-544.9982130
TCGCGCG403.4449658E-739.3734369
TATGCCG700.038.56989743
TATATCT301.1376573E-437.4985134
CGCTATA301.1376573E-437.4985131
AACCGTT250.002104401435.9985719
CGTATGC751.8189894E-1235.9985741
TCAGATA250.002104401435.998572
GACGCTA250.002104401435.9985729
ATAACAC250.002104401435.998573
TATCTCG702.0008883E-1135.3557436
CGCGCGC458.666775E-734.9986110
TATAGGT458.666775E-734.998613
CAAATAT606.3919288E-933.7486623
CTCGTAT801.8189894E-1233.74865739
TCTCGTA801.8189894E-1233.74865738
TCGTATG801.8189894E-1233.74865740
GCGCGCT551.12215275E-732.72597511
GATACCG352.81482E-432.1415825
ACGAGTC352.81482E-432.1415825
Produced by FastQC (version 0.11.2)