FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_80.34100000009df8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_80.34100000009df8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869437
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC22750.2616635822952095No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3500.039.85571745
ATGCCGT4250.032.82235744
TATGCCG4450.031.34719343
GCCCACG4550.031.15273318
CTGCGCG300.00514747829.9989269
TGCGCGC300.00514747829.99892610
CCCACGA4850.029.6896619
CGTATGC4750.029.36737341
CCGAGCC4850.029.22575814
GCGAATC4400.029.14668533
GGCGAAT4400.029.14668532
CAAGGCG4450.028.81919329
CGAGCCC4950.028.6353415
GTATGCC4950.028.18080942
AGCCCAC5050.028.06830217
CGAGACC4750.026.99903523
CTCGTAT5300.026.74432839
GAGCCCA5400.026.66571416
CCACGAG5350.026.4943820
AGGCGAA4850.026.44235231
Produced by FastQC (version 0.11.2)