FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_8.341000000098c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_8.341000000098c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322401
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT6600.20471400522951233No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC4660.14454049460144353No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC4620.14329980366065861No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATC4080.12655047596006216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATT301.1385628E-437.4974174
CGTATGC504.86325E-835.99751741
GCCCACG551.12411726E-732.72501818
GGCGAAT551.12411726E-732.72501832
GTATATA300.005066003730.0912671
GTACATG1450.029.572451
ACTCGGG406.1589293E-428.12306241
GGGGCAA1053.6379788E-1227.8552218
TATGCCG654.8580114E-727.690443
TCGTATG654.8580114E-727.690440
ATGCCGT654.8580114E-727.690444
AGGACCT755.6164936E-826.998145
CACGAGA709.2783375E-725.71251321
CGTCGGG1201.6370905E-1124.37332214
CATGCTA1301.8189894E-1224.22914
TACATGC1500.023.9983462
GTATGCC751.6923514E-623.99834642
CTGTACT851.8861283E-723.8218884
TACCCGG1252.910383E-1123.3983886
AGGCCTC1252.910383E-1123.39838830
Produced by FastQC (version 0.11.2)