FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_79.34100000009deb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_79.34100000009deb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995009
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC71730.7208980019276208No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT14410.1448228106479439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7650.041.46924645
ATGCCGT9500.033.1568244
CGAGACG9750.031.6143623
GCCCACG10300.030.79997618
TATGCCG10300.030.58153543
AGCCCAC10450.030.57317417
CCCACGA10500.030.21330819
CGAGCCC10450.030.14256515
TCGCCCC453.4911493E-529.9990325
CCGAGCC10600.029.71601914
TAACCTA10550.029.43032631
CGTATGC10750.029.30137641
ACGAGAC10750.029.30137622
TATCTCG10800.028.95739436
CCACGAG11100.028.58015420
CACGAGA11250.028.19908521
AACCTAT11050.028.09863932
GTATGCC11250.027.99909242
GAGCCCA11250.027.99909216
TCCGAGC11350.027.75240313
Produced by FastQC (version 0.11.2)