FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_78.34100000009dde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_78.34100000009dde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964674
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC59120.6128495222220148RNA PCR Primer, Index 46 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGT12380.12833350955866957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7100.041.5128945
GCCCACG7950.037.35741418
CGAGCCC8100.036.66560715
ATGCCGT8100.036.3878444
CCGAGCC8250.036.27168314
CATTCCG8050.036.05486329
CCCACGA8250.035.99896219
TATGCCG8200.035.94408843
CGTATGC8300.035.51102441
GTATGCC8350.035.29838642
CCGAATC8300.035.2399533
ATTCCGA8600.034.01064730
GAGCCCA8800.033.74902716
CCACGAG8800.033.74902720
AGCCCAC8850.033.55835717
TCCGAAT8950.032.68062232
AATCTCG9100.032.6364236
TCCGAGC9150.032.4580813
TTCCGAA9150.031.96629131
TCGTATG9250.031.86394940
Produced by FastQC (version 0.11.2)