FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_77.34100000009dc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_77.34100000009dc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137033
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC35270.31019328374814104No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT13050.11477239446876211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4300.036.10348545
CCCACGA5150.030.58153519
GCCCACG5300.029.71602418
ATGCCGT5300.029.71602444
TGCGACC5000.029.69904329
GCGACCT4950.029.54450230
GACTGCG5100.029.11670726
CGACCTA5150.028.3971431
CTGCGAC5250.027.85624528
CGGGGCA654.8746915E-727.69141617
TATGCCG5650.027.4769943
AGCCCAC5750.027.39042317
CCACGAG5900.027.07539720
CGAGCCC5900.026.69405415
GACCTAT5650.025.8841232
CCGAGCC6100.025.8188414
ACTGCGA5900.025.55002427
GTATGCC6350.024.8023542
CTCCGAG6450.024.76664212
AGACTGC6300.024.28493125
Produced by FastQC (version 0.11.2)