FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_76.34100000009db4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_76.34100000009db4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236716
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC27790.2247080170386734No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCCG253.8916194E-544.99874529
TGCCGTC4600.032.2817145
CGCGCGC352.8209612E-432.14196410
TCCGCGA300.00514810829.99916616
GCGCCTC406.1674154E-428.12421611
GTCCGGG406.1674154E-428.12421631
ATGCCGT5300.028.01808744
GCCCACG5250.027.85636718
AGCCCAC5300.027.16905617
TATGCCG5400.027.08257943
CCCACGA5650.026.28245419
CGAGCCC5600.026.11534515
CGAGACC5050.025.84086223
GCGCGCT709.310752E-725.7135711
CCGAGCC5700.025.6571814
GTATGCC5850.024.99930442
TCGCGCG450.00122680724.9993049
TCCGGGC450.00122680724.99930432
CGTATGC5950.024.57914741
ACAGCGC551.3685439E-424.544778
Produced by FastQC (version 0.11.2)