FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_75.34100000009da8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_75.34100000009da8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1207302
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC86800.7189584710370728No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT26680.2209886175952661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10650.041.4066245
ATGCCGT13350.033.2007844
TATGCCG14200.031.2134143
GTATGCC14600.030.5123542
GCCCACG14600.030.35824618
CCCACGA14700.030.30478319
GGTCGCG453.492041E-529.9986747
CGAGACT14550.029.99867423
CGTATGC14900.029.74700741
ACGAGAC15150.029.40464222
CACGAGA15250.029.3593621
AGCCCAC15050.029.30103117
TATCTCG14600.029.27952836
CCGAGCC15200.029.01187514
AGACTAG14750.028.82923725
CGAGCCC15350.028.7283715
CCACGAG15650.028.60896120
GACTAGA15350.028.2886526
CGCGCGC406.1679195E-428.12375610
TCGTATG15900.028.0176340
Produced by FastQC (version 0.11.2)