FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_74.34100000009d9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_74.34100000009d9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460804
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC10500.2278626053593285TruSeq Adapter, Index 12 (95% over 21bp)
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG8070.17512868811902674No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGGC7060.1532104756035104No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTC4790.10394875044487462No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA4760.10329771442956223No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGGA4610.1000425343530004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT2100.036.42795623
GCCCACG2150.034.534318
ATGCCGT2200.033.7494344
TATGCCG2300.033.26030743
GTATGCC2300.033.26030742
CCCACGA2250.032.99944319
TCGTATG2400.031.8744640
CGTATGC2450.031.22396341
CTCGTAT2450.031.22396339
CTCCGAG2550.030.88183412
GCCTCGA1650.029.99949332
ACGAGAC2650.028.86743722
CACGAGA2600.028.5572121
TACCCGG1900.028.4205726
GGGGCAA1750.028.28523618
CGTCGGG1850.027.97250214
AGCCAGG1800.027.49953726
GAGCCAG1850.026.75630625
GTCCTAA608.130584E-626.269511
GCCAGGC1900.026.05219327
Produced by FastQC (version 0.11.2)