Basic Statistics
Measure | Value |
---|---|
Filename | H23V3BCXX l02n01 kb2_1_73.34100000009d71.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1424 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC | 61 | 4.283707865168539 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC | 54 | 3.7921348314606744 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC | 37 | 2.598314606741573 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC | 22 | 1.544943820224719 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT | 21 | 1.4747191011235954 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT | 12 | 0.8426966292134831 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT | 8 | 0.5617977528089888 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT | 8 | 0.5617977528089888 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC | 6 | 0.42134831460674155 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC | 5 | 0.35112359550561795 | TruSeq Adapter, Index 12 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG | 4 | 0.2808988764044944 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT | 3 | 0.21067415730337077 | No Hit |
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAA | 2 | 0.1404494382022472 | No Hit |
GGCTTGGAAGCAGCCATCCTTTGAAGAAAGCGTAATAGCTCACTGGTCTAG | 2 | 0.1404494382022472 | No Hit |
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC | 2 | 0.1404494382022472 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 30 | 9.867254E-7 | 44.999996 | 45 |
ATGCCGT | 35 | 2.8606937E-6 | 38.571426 | 44 |
AGCCCAC | 40 | 7.1789163E-6 | 33.75 | 17 |
GTATGCC | 40 | 7.1789163E-6 | 33.75 | 42 |
ATCTCGT | 40 | 7.1789163E-6 | 33.75 | 37 |
ATCTCCG | 40 | 7.1789163E-6 | 33.75 | 10 |
TATGCCG | 40 | 7.1789163E-6 | 33.75 | 43 |
GAGCCCA | 40 | 7.1789163E-6 | 33.75 | 16 |
TCTCGTA | 40 | 7.1789163E-6 | 33.75 | 38 |
CGTATGC | 40 | 7.1789163E-6 | 33.75 | 41 |
CGAGCCC | 40 | 7.1789163E-6 | 33.75 | 15 |
CCGAGCC | 40 | 7.1789163E-6 | 33.75 | 14 |
TCGTATG | 40 | 7.1789163E-6 | 33.75 | 40 |
TCTCCGA | 40 | 7.1789163E-6 | 33.75 | 11 |
TCCGAGC | 40 | 7.1789163E-6 | 33.75 | 13 |
CTCGTAT | 40 | 7.1789163E-6 | 33.75 | 39 |
CTCCGAG | 40 | 7.1789163E-6 | 33.75 | 12 |
GCCCACG | 35 | 1.6340625E-4 | 32.142857 | 18 |
CCACGAG | 35 | 1.6340625E-4 | 32.142857 | 20 |
CACGAGA | 35 | 1.6340625E-4 | 32.142857 | 21 |