FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_73.34100000009d71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_73.34100000009d71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC614.283707865168539No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC543.7921348314606744No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC372.598314606741573No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC221.544943820224719No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT211.4747191011235954No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT120.8426966292134831No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT80.5617977528089888No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT80.5617977528089888No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC60.42134831460674155No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC50.35112359550561795TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG40.2808988764044944No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT30.21067415730337077No Hit
CCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAA20.1404494382022472No Hit
GGCTTGGAAGCAGCCATCCTTTGAAGAAAGCGTAATAGCTCACTGGTCTAG20.1404494382022472No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC20.1404494382022472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC309.867254E-744.99999645
ATGCCGT352.8606937E-638.57142644
AGCCCAC407.1789163E-633.7517
GTATGCC407.1789163E-633.7542
ATCTCGT407.1789163E-633.7537
ATCTCCG407.1789163E-633.7510
TATGCCG407.1789163E-633.7543
GAGCCCA407.1789163E-633.7516
TCTCGTA407.1789163E-633.7538
CGTATGC407.1789163E-633.7541
CGAGCCC407.1789163E-633.7515
CCGAGCC407.1789163E-633.7514
TCGTATG407.1789163E-633.7540
TCTCCGA407.1789163E-633.7511
TCCGAGC407.1789163E-633.7513
CTCGTAT407.1789163E-633.7539
CTCCGAG407.1789163E-633.7512
GCCCACG351.6340625E-432.14285718
CCACGAG351.6340625E-432.14285720
CACGAGA351.6340625E-432.14285721