FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_72.34100000009d64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_72.34100000009d64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154457
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.1592676278834886No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1670.108120706733913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.10423613044407182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTAAC301.1357856E-437.4975711
GTACTAA352.7624884E-432.234691
CGTCCTG300.005135260529.99805642
ACTGCTC406.144136E-428.123188
AGGCCAA450.001222213424.9983827
AATAATG500.002256661222.4985455
TACACAC702.6848196E-522.4985435
CGGGTGG754.5718756E-520.998644
CTTAGAT654.2172297E-420.7678873
GTACAAT550.003922836420.453226
AGGAAAT550.003922836420.4532224
CAATACA706.9491094E-419.2844644
ACACAAC600.006488026618.7487856
TTTTGGA600.006488026618.74878521
AATACAT600.006488026618.7487855
TACGGGT1002.0291918E-517.9988352
AGTACCG750.001104799617.9988355
TCTTAGA1004.140035E-415.74898052
ACGGGGC1004.140035E-415.74898053
TACGGGG3100.015.2409492
Produced by FastQC (version 0.11.2)