FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_71.34100000009d58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_71.34100000009d58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246918
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC276062.2139386872272278No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT66200.5309090092532147No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG21360.17130236310647534No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA19940.15991428466025834No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT18300.14676185603223307No Hit
CCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATG17660.14162920095788176No Hit
CTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG17550.14074702586697763No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC17250.13834109380087542No Hit
AACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAG16230.13016092477612803No Hit
TACTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTA12950.1038560675200775No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC31250.042.98254445
ATGCCGT37500.035.69878844
TATGCCG39950.033.6221443
TATCTCG40200.033.18917536
GTATGCC40550.033.1246542
CGTATGC40450.033.09529541
TCGTATG40800.032.8113940
CGAGACA40100.032.7108623
CTCGTAT40800.032.64595439
TCTCGTA41200.032.32900238
CCACGAG41600.032.2885720
CACGAGA41700.032.26509521
ACGAGAC41950.032.1800822
ATAATAT41700.031.72554632
CCCACGA42500.031.60481319
CATAATA42100.031.47755631
TAATATC41850.031.39678633
GCCCACG43400.030.94941318
ACATAAT42900.030.83811830
ATCTCGT44150.030.37269837