FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_70.34100000009d4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_70.34100000009d4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1358623
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC72050.5303163570762456No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT16620.12232974121592229No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8600.039.7659845
GCCCACG9850.034.4911218
TATGCCG10000.033.9737543
CCCACGA10150.033.47167619
ATGCCGT10200.033.307644
GTATGCC10400.032.6670742
AGCCCAC10650.031.90023417
GACTTGC10300.031.45515426
CGAGACT10950.030.61530923
CCGAGCC11150.030.4697314
CGTATGC11300.030.26437441
CGAGCCC11500.029.54238915
CTCGTAT11350.029.33812739
CCACGAG11750.029.1053120
GAGCCCA11700.029.03739216
CCGTGCA903.965397E-1027.498999
TCCGAGC12450.027.46886813
TCTCGTA12650.026.67886238
TTGCTGG12400.026.1280729
CACGAGA13150.026.00664921
Produced by FastQC (version 0.11.2)