FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_7.341000000098ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_7.341000000098ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810908
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC9490.11702930542059026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2000.026.99877744
TGCCGTC2000.026.99877745
GCCCACG2050.026.34027318
CGTATGC2100.025.71312341
TATGCCG2100.025.71312343
CCCGGCT450.001226566524.99886915
CCCACGA2200.024.54434419
GCGGTAC1450.023.2748142
GTATGCC2350.022.97768442
GTAGTAT602.4336083E-422.5448571
CGCGGTA1500.022.49898341
TACCTCG1500.022.49898336
TCGCGGT1650.021.81719640
GTACCGC1459.094947E-1221.7231566
ACCGCGA1459.094947E-1221.7231568
TACCGCG1459.094947E-1221.7231567
CGAGACG2500.021.59902423
CACGAGA2550.021.17551221
CCGTTAC2350.021.1058241
CGAGGTA1601.8189894E-1221.09279612
Produced by FastQC (version 0.11.2)