FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_69.34100000009d3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_69.34100000009d3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572285
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC169711.0793844627405336No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGT31300.1990733232206629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC18050.042.38062345
GCCCGTT250.00210774635.998621
ATGCCGT22250.034.38068444
GCCCACG23000.033.357418
CGCTATA22750.033.13057731
TATGCCG23350.032.857443
CGACGCT23050.032.6017728
GACGCTA23050.032.6017729
CCCACGA23550.032.5783519
ACGACGC23250.032.41809527
GTATGCC23700.032.27722542
AGCCCAC23850.032.07422617
ACGCTAT23700.031.8025630
CCACGAG24250.031.73072620
TATCTCG24300.031.66543636
CGTATGC24150.031.58262441
CTCGTAT24300.031.57284739
CGAGCCC24300.031.48025715
CGAGACG24150.031.39629623
CCGAGCC24750.031.08969914
Produced by FastQC (version 0.11.2)