FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_68.34100000009d21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_68.34100000009d21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188434
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC43270.36409257897367464No Hit
CTTCTATTCGAAGTGCTTTTCACCTTTCCTTCACAGTACTTGTTCACTATC12690.10677917326498568No Hit
GTTTCAGGTTCTATTTCACCCTTCTATTCGAAGTGCTTTTCACCTTTCCTT12210.10274024472541177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6750.036.99837545
GCCCACG7000.035.67700218
AGCCCAC7150.034.6138617
ATGCCGT7300.034.21082744
GCGAATC6250.034.19849433
GGCGAAT6300.033.9270832
TATGCCG7400.033.74851643
CGAGCCC7400.033.74851615
CCCACGA7400.033.44447719
CCGAGCC7600.033.15643714
GAGCCCA7650.032.64562616
GTATGCC7850.032.100542
CAAGGCG6750.031.66527629
TCCGAGC8050.031.5824613
CGTATGC8000.031.21737741
CGAGACC6800.031.10157623
CTCCGAG8450.030.08743512
ACGAGAC8250.029.9986822
CCACGAG8300.029.81796820
AGGCGAA7500.029.0987231
Produced by FastQC (version 0.11.2)