FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_67.34100000009d14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_67.34100000009d14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327194
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC120910.9110197906259372No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT25570.19266211269791755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13200.043.12341345
ATGCCGT16900.033.6821944
GCCCACG17400.033.23153318
CCGAGCC17400.033.23153314
CGAGCCC17400.033.23153315
TATGCCG17200.033.2255243
AGCCCAC17700.032.6682917
CCCACGA17800.032.4847619
GAGCCCA17950.032.213316
CCACGAG18300.031.59719820
CGAGACG17950.031.5865823
TAACCTA17950.031.08520531
GTATGCC18500.031.0123742
CGTATGC18650.030.88358141
ACCTATC18150.030.86663433
AACCTAT18150.030.6187132
TATCTCG18900.030.35602436
CGATAAC18600.030.11985628
TCCGAGC19250.030.03785313
CACGAGA19450.029.72898121
Produced by FastQC (version 0.11.2)