FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_66.34100000009d07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_66.34100000009d07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247692
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC20500.16430336974189141RNA PCR Primer, Index 46 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3800.037.89328845
GCCCACG3850.036.81677218
ATGCCGT3900.036.3447644
CGTATGC4050.034.9986641
TATGCCG4200.034.284443
CCCACGA4150.034.15531519
CCGAGCC4200.033.7487114
GTATGCC4350.033.1021842
CTCCGAG4450.031.85271312
CGAGCCC4450.031.85271315
CGAGACC4150.031.44457623
CCACGAG4650.030.96655720
TCCGAGC4750.029.84096113
GAGCCCA4900.028.92746416
CACGAGA5000.028.79889921
AGCCCAC4950.028.63526717
CCGGGTA406.1678246E-428.12392237
TCGCGCG406.1678246E-428.1239229
ACGAGAC5100.027.79305522
CTCGTAT5600.026.51684239
Produced by FastQC (version 0.11.2)