FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_65.34100000009cf9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_65.34100000009cf9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882356
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC8981310.178771380259215No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT203392.3050786757272577No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCG69570.7884572666814755No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCT12650.14336616966394516No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTAGCTAAAGG10450.11843292276586775No Hit
CTACTAACGGGGTCTCGGTTGATTTCCCTTCCTTTAGCTACTTAGATGTTT9350.10596629931682904No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAACATCTAAGTCTGTCTCTT9300.1053996346146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC94100.044.5916145
ATGCCGT114850.036.5352244
TCGACCA250.002107286635.9984629
TATGCCG122150.034.38862243
CGTATGC122100.034.36585241
TATCTCG121700.034.23847236
ATCTCGT121850.034.2332537
CGAGACT123300.034.21386323
GCCCACG122850.034.2109918
GTATGCC122800.034.206642
CCCACGA123100.034.19634219
GCGACCT122500.034.1801730
ACGAGAC123300.034.1773722
TGCGACC123000.034.15097829
GACTGCG123600.034.14902526
GACCTAT122650.034.12002632
CACGAGA123400.034.11320521
TCTCGTA122350.034.11173638
CTCGTAT122550.034.11114539
CGACCTA122850.034.10110531
Produced by FastQC (version 0.11.2)