FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_64.34100000009cec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_64.34100000009cec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599597
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC31410.1963619586683396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5000.039.59848445
ATGCCGT5850.033.8448644
GCCCACG6000.032.99873718
CCGAGCC6200.031.93426514
CCCACGA6400.031.28786719
TATGCCG6450.031.04532443
CGAGCCC6400.030.93631715
CGTATGC6800.029.44740541
AGCCCAC7000.028.28463417
CCACGAG7100.027.8862620
TCCGAGC7250.027.30930113
TCGTATG7350.027.24385640
GTATGCC7450.026.87816842
TCGCCCC608.196603E-626.24899525
CGGGACG608.196603E-626.24899517
CTCCGAG7800.025.67209412
GAGCCCA7850.024.93535216
ACGAGAC8150.024.56961422
TCCGCGA551.3688848E-424.54451616
CCGCGAA551.3688848E-424.54451617
Produced by FastQC (version 0.11.2)