FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_63.34100000009cdf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_63.34100000009cdf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012917
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC54420.5372602098691206No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT11230.11086791908912577No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7800.040.96005645
GCATCCG250.00210736535.99869537
ATGCCGT8900.035.64477544
TATGCCG8950.035.44564443
CTCGTAT9100.035.1086239
CGTATGC9150.034.91676741
ACGAGAC9250.034.5392922
CCACGAG9250.034.5392920
CGAGACT8800.034.51579723
CCCACGA9300.034.35359619
CACGAGA9400.033.98813221
GACTGCG1200.033.74877526
GCCCACG9500.033.63036318
ATCTCGT9550.033.4542937
TCGTATG9550.033.4542940
TATCTCG9300.033.38588736
TCTCGTA9600.033.28004538
GTATGCC9800.032.6008642
CTGCGAC1250.032.39882728
CGAGACG352.8207415E-432.14169723
Produced by FastQC (version 0.11.2)