FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_62.34100000009cc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_62.34100000009cc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792455
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC8110.10234019597327293TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGCG1350.033.33239726
TATGCCG2800.032.14195643
GCCCACG2800.031.33840818
CCCACGA3000.029.2491819
CGTATGC3100.029.03144341
TCGTATG3150.028.57062540
ATGCCGT3200.028.12421244
GTATGCC3450.026.7383842
CTCGTAT3500.025.71356639
CTGCGAC1750.025.71356628
GGTATCA4050.025.0308861
CCGAGCC3650.024.65684314
CCTAGAC551.3679393E-424.5447653
ACTGCGA1900.023.68354627
TGCGACC1900.023.68354629
GTATCAA10700.023.1594181
ACCTATC2050.023.04813633
TGCCGTC4050.022.7771445
CCACGAG4000.022.49936920
CTCCGAG4050.022.221612
Produced by FastQC (version 0.11.2)