FastQCFastQC Report
Wed 6 May 2015
H23V3BCXX l02n01 kb2_1_61.34100000009ca9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23V3BCXX l02n01 kb2_1_61.34100000009ca9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535960
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC13110.2446078065527278RNA PCR Primer, Index 27 (95% over 21bp)
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTA5560.10373908500634377No Hit
GCTTTAGATTATCCTTGAAAACTTCGAACCTAAGAAACTTCTCTTCAACGG5460.10187327412493469No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCTA301.1393456E-437.49867627
TATGCCG2500.035.9987343
CCCACGA2550.035.29287319
GTATGCC2600.034.61416642
ATGCCGT2650.033.1120444
TATCTCG2850.032.3672836
CGTATGC2800.032.14172441
GCCCACG2850.031.57783318
TCGTATG2900.031.03338840
CGAGACG2400.030.93640923
CTCGTAT3000.030.74891739
ACGAGAC2900.030.25755322
CGTCCCT300.005145723529.99894127
GCACTGA453.488244E-529.9989416
CACGAGA2950.029.74471521
TCTCGTA3050.029.50715638
TGCCGTC3000.029.24896845
CGCCAGT702.8810973E-828.92755139
CCACGAG3200.028.82710620
CCGTTAC1200.028.1686821
Produced by FastQC (version 0.11.2)